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Smac mimetic

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Smac mimetic is a synthetic compound designed to mimic the function of the Smac (second mitochondria-derived activator of caspase) protein. Smac mimetic acts as an antagonist of the inhibitor of apoptosis (IAP) proteins, which are involved in regulating programmed cell death. The core function of Smac mimetic is to promote the activation of caspases, the enzymes responsible for executing the apoptotic process.

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Lab products found in correlation

Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) Ovalbumin (ova), by Merck Group (1 mentions) Collagenase 4, by Merck Group (1 mentions) Dnase 1, by Roche (1 mentions) Siinfekl, by Thermo Fisher Scientific (1 mentions) Collagenase d, by Roche (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Pe annexin 5 apoptosis detection kit 1, by BD (1 mentions) Mouse il 10 elisa kit, by ABclonal (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) 3 methyladenine 3 ma, by Merck Group (1 mentions) Rabbit anti flag antibody, by Cell Signaling Technology (1 mentions) Mouse anti ha antibody, by Santa Cruz Biotechnology (1 mentions) Fluo 4, by Thermo Fisher Scientific (1 mentions) Ym201636, by InvivoGen (1 mentions)

2 protocols using smac mimetic

1

Apoptosis Induction and Immune Response

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Collagenase D (Cat#1108888200) and DNase I (Cat#1010415900) were purchased from Roche. Collagenase IV (Cat#C4-BIOC), Ovalbumin (Cat#S7951) and anti-chicken egg albumin (Ovalbumin) (Cat#C6534) were from Sigma. Ovalbumin-Alexa FluorTM 488 (Cat#O34781), Ovalbumin-Alexa Fluor 647TM (Cat#O34784), Alexa FlourTM 647 NHS Ester (Cat#A37573), CFSE (Cat#C34554), and SIINFEKL were from Invitrogen. Diphenyleneiodonium chloride (HY-100965) was from MEC. The expression plasmid for mCherry:Galectin-3 (Cat#85662) was purchased from Addgene. PE Annexin V Apoptosis Detection Kit I was purchased from BD bioscience (Cat#559763). Mouse IL-10 ELISA Kit was from ABclonal (Cat#RK00016). TNF-α was purchased from eBioscience. Z-VAD-FMK was from Calbiochem and Smac mimetic was from APExBIO (Cat#SM164).
Wang Y., Zhang Q., He T., Wang Y., Lu T., Wang Z., Wang Y., Lin S., Yang K., Wang X., Xie J., Zhou Y., Hong Y., Liu W.H., Mao K., Cheng S.C., Chen X., Li Q, & Xiao N. (2023). The transcription factor Zeb1 controls homeostasis and function of type 1 conventional dendritic cells. Nature Communications, 14, 6639.
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2

Analyzing Cell Death Pathways

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Mouse TNF was purchased from eBioscience. Nigericin (Nig) and Hoechst 33342 were obtained from Invitrogen. Smac mimetic was from APExBIO. Ultrapure lipopolysaccharides (LPS), 4-hydroxytamoxifen (4-OHT), propidium iodide (PI), SF1670 and 3-methyladenine (3MA) were purchased from Sigma-Aldrich. YM201636 was provided by Invivogen. PITenin-7 and z-VAD were acquired from Calbiochem. All ion indicators including Asante NaTRIUM Green-2 (ANG-2) and Asante Potassium Green-2 (APG-2) were provided by TEFLabs, except that Fluo-4 was from Invitrogen. Antibodies were from commercial sources: Rabbit anti-Flag antibody (14793S, Cell Signaling), Mouse anti-HA antibody (sc-7392, Santa Cruz) and Mouse anti-GST antibody (M20007, Abmart).
Chen X., He W.T., Hu L., Li J., Fang Y., Wang X., Xu X., Wang Z., Huang K, & Han J. (2016). Pyroptosis is driven by non-selective gasdermin-D pore and its morphology is different from MLKL channel-mediated necroptosis. Cell Research, 26(9), 1007-1020.
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