Male C57BL/6NCrSlc mice were purchased from Japan SLC (Shizuoka, Japan). Seven-week-old mice with/without treatment with 44 mg/kg body weight busulfan (Sigma-Aldrich, St Louis, MO, USA) at 3 weeks of age, which depletes germ cell in testes, were used as recipients. Male C57BL/6 Tg14(act-EGFP)OsbY01 ‘green’ mice were used as testis donors57 (link). Testis pieces collected from newborn mice (1.5–4.5 days postpartum) were transplanted into recipient testes. Briefly, recipient mice were anesthetised with isoflurane (FUJIFILM Wako Pure Chemical Co., Ltd., Osaka, Japan) and a small cut was made in the tunica albuginea of each animal using fine forceps. A single testis piece was inserted into the testicular parenchyma25 (link). In some cases, recipient testes were treated five times with all-trans retinoic acid (750 µg per mouse, Sigma-Aldrich) dissolved in a 10% ethanol-sesame oil (Nacalai Tesque, Kyoto, Japan) with 8.5-day intervals just prior to sacrifice (Fig. 1 ).
Ethanol sesame oil
Manufactured by Nacalai Tesque
Sourced in Japan
Ethanol-sesame oil is a mixture of ethanol and sesame oil. It is a clear liquid solution.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ethanol sesame oil
1
Testis Transplantation in Busulfan-Treated Mice
2
Diverse Mouse Models for Reproductive Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Male and female C57BL/6NCrSlc (B6) mice at 8 or 52 weeks of age, and male WBB6F1/kit-KitW/KitW-v/Slc (W) mice at 8 weeks of age were
purchased from Japan SLC (Shizuoka, Japan). At 7 days of age, male B6 mice were obtained by intercrossing B6 mice. For busulfan treatment, male B6 mice at 4 or 6 weeks of age were injected
intraperitoneally with 44 or 15 mg/kg body weight busulfan, respectively (Sigma-Aldrich, St Louis, MO, USA). Experimental cryptorchidism was performed on 8-week-old male B6 mice, as
described by Nishimune et al. [25 (link)]. Hypophysectomized, thyroidectomized, adrenalectomized, and sham-operated B6 mice were also
purchased from Japan SLC; these operations were conducted at 6 weeks of age. For retinoic acid (RA) treatment, all-trans RA (Sigma-Aldrich) was dissolved in a 10% ethanol-sesame oil (Nacalai
Tesque, Kyoto, Japan) solution at 3.75 mg/ml and then injected (200 μl) intraperitoneally into 8-week-old B6 mice (750 μg per mouse). All animal experiments were approved by the
Institutional Animal Care and Use Committee of Shinshu University (Approval No. 260013 and No. 280120).
purchased from Japan SLC (Shizuoka, Japan). At 7 days of age, male B6 mice were obtained by intercrossing B6 mice. For busulfan treatment, male B6 mice at 4 or 6 weeks of age were injected
intraperitoneally with 44 or 15 mg/kg body weight busulfan, respectively (Sigma-Aldrich, St Louis, MO, USA). Experimental cryptorchidism was performed on 8-week-old male B6 mice, as
described by Nishimune et al. [25 (link)]. Hypophysectomized, thyroidectomized, adrenalectomized, and sham-operated B6 mice were also
purchased from Japan SLC; these operations were conducted at 6 weeks of age. For retinoic acid (RA) treatment, all-trans RA (Sigma-Aldrich) was dissolved in a 10% ethanol-sesame oil (Nacalai
Tesque, Kyoto, Japan) solution at 3.75 mg/ml and then injected (200 μl) intraperitoneally into 8-week-old B6 mice (750 μg per mouse). All animal experiments were approved by the
Institutional Animal Care and Use Committee of Shinshu University (Approval No. 260013 and No. 280120).
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