All solvents and starting materials were purchased commercially (TCI Shanghai, J&K, Sigma-Aldrich) and used without further purification. The TCO-NHS was commercially available from Click Chemistry Tools. The GEBP11 peptide was supplied by the State Key Laboratory of Cancer Biology, the Institute of Digestive Diseases, Xijing Hospital, and the Fourth Military Medical University.
The high-performance liquid chromatography (HPLC) was used for purification of probes by on a Waters prep LC 2545 instrument. ESI-TOF-MS spectra measurements were performed by a Bruker QTOF II mass spectrometer. The imaging experiments in vitro were recorded on an Olympus FV 10i confocal fluorescent microscope. In vivo fluorescence imaging analysis was carried out in an IVIS Kinetic imaging system. The binding affinity was detected with a BD Accuri C6 flow cytometry.
A detailed description of the synthesis and characterization of all compounds can be found in theSupplementary Materials .
The high-performance liquid chromatography (HPLC) was used for purification of probes by on a Waters prep LC 2545 instrument. ESI-TOF-MS spectra measurements were performed by a Bruker QTOF II mass spectrometer. The imaging experiments in vitro were recorded on an Olympus FV 10i confocal fluorescent microscope. In vivo fluorescence imaging analysis was carried out in an IVIS Kinetic imaging system. The binding affinity was detected with a BD Accuri C6 flow cytometry.
A detailed description of the synthesis and characterization of all compounds can be found in the