Wst 1 solution

Cell viability was measured using the WST-1 assay. Briefly, cells were seeded in 96-well plates at 4,000 cells per well, and treated with different concentrations of 5-Aza in triplicate for an indicated period of time. At the end of the treatment, 20 μl of WST-1 solution (Beyotime Biotechnology, Shanghai, China) was added into each well and the cells were cultured at 37°C for an additional 2 h. Finally, the absorbance was measured at 450 nm and 690 nm using a microplate reader (Thermo Fisher Scientific, Vantaa, Finland). The data were calculated by optical density (OD) OD450-OD690 and represented the cell viability.

Cell viability was assessed using the WST-1 assay. Cells were pretreated with different concentrations of CK for 24 h and then exposed to various concentrations of TRAIL for 24 h. The medium was then removed, and 10 μl per well of the WST-1 solution (Beyotime Biotechnology, Jiangsu, China) was added. After 4-h incubation, the absorbance was measured at 450 nm using a microplate reader (Bio-Rad, Hercules, CA, USA). All experiments were performed in triplicate and repeated at least three times. Cell viability is expressed as the percentage of the control, which was set to 100%.