As recommended by the Clinical and Laboratory Standards Institute, an antifungal susceptibility test was conducted using the broth dilution testing reference method M27-A3 (40 , 41 (link)). HAL-ES and ES were tested using a 0.03 to 15% ethanol concentration range. Fluconazole (0.125 to 64 μg/mL; Macklin, Shanghai, China) was included as a positive control. The MIC was defined as the lowest concentration that showed no visible fungal growth and turbidity after 24 to 48 h of incubation. We assessed the growth of C. albicans 36 h after treatment under an inverted microscope (IX73; Olympus, Tokyo, Japan). Image acquisition was conducted using cellSens Dimension software (Olympus). Subculturing of 100 μL of the culture from each optically clear well was performed on YPD plates at 37°C for 24 to 48 h to obtain the MFC, the lowest drug concentration at which there was no fungal growth. Experiments were performed three times independently.
Fluconazole
Manufactured by Maclin Biochemical Technology
Sourced in China
Fluconazole is a laboratory product manufactured by Maclin Biochemical Technology. It is a white crystalline powder that is soluble in water and alcohol. Fluconazole is used as a reference standard in the analysis and characterization of pharmaceutical and biochemical samples.
Automatically generated - may contain errors
Lab products found in correlation
Cellsens dimension software, by Olympus (2 mentions)
Uridine diphosphate glucuronic acid, by Merck Group (1 mentions)
Metronidazole, by Maclin Biochemical Technology (1 mentions)
Tetracycline, by Maclin Biochemical Technology (1 mentions)
Chloramphenicol, by Maclin Biochemical Technology (1 mentions)
Arginine, by Maclin Biochemical Technology (1 mentions)
Clarithromycin, by Maclin Biochemical Technology (1 mentions)
Potassium dihydrogen phosphate, by Loba Chemie (1 mentions)
Methanol, by BD (1 mentions)
Methylparaben, by Loba Chemie (1 mentions)
Propylparaben, by Loba Chemie (1 mentions)
Hydrochloric acid (hcl), by Loba Chemie (1 mentions)
5 protocols using fluconazole
1
Antifungal Susceptibility Testing of Candida albicans
2
Antifungal Susceptibility Testing of Candida albicans
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
As recommended by the Clinical and Laboratory Standards Institute, an antifungal susceptibility test was conducted using the broth dilution testing reference method M27-A3 (40 , 41 (link)). HAL-ES and ES were tested using a 0.03 to 15% ethanol concentration range. Fluconazole (0.125 to 64 μg/mL; Macklin, Shanghai, China) was included as a positive control. The MIC was defined as the lowest concentration that showed no visible fungal growth and turbidity after 24 to 48 h of incubation. We assessed the growth of C. albicans 36 h after treatment under an inverted microscope (IX73; Olympus, Tokyo, Japan). Image acquisition was conducted using cellSens Dimension software (Olympus). Subculturing of 100 μL of the culture from each optically clear well was performed on YPD plates at 37°C for 24 to 48 h to obtain the MFC, the lowest drug concentration at which there was no fungal growth. Experiments were performed three times independently.
3
Chemical Analysis of Drug Purity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Estradiol (E2, purity > 98%), testosterone (purity ≥ 98%), entacapone (purity ≥ 98%), and nilotinib (purity ≥ 99%) were purchased from Aladdin Co., Ltd. (Shanghai, China). Trifluoperazine (TFP, purity ≥ 99%), fluconazole (purity ≥ 98%), and niflumic acid (purity ≥ 98%) were purchased from Macklin Co., Inc. (Shanghai, China). Magnolol (MAG, purity ≥ 98%) and hecogenin (purity ≥ 98%) were purchased from Pufei De Biotech Co., Ltd. (Chengdu, China). 4-Nitrophenol (pNP, purity > 99%), 4-nitrophenol-β-O-glucuronide (pNPG, purity > 98%), and 3′-azido-3′-deoxythymidine (purity ≥ 98%) were purchased from J&K Chemical Ltd. (Shanghai, China). Uridine diphosphate glucuronic acid (UDPGA, purity > 96%) and E. coli β-glucuronidase (GUS, Cat No. G8295, 25KU/2.1 mg) were purchased from Sigma-Aldrich (Shanghai, China).
4
Preparation of Chemical Solutions for Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Arginine (Arg, 98%), chloramphenicol (CAP, 98%), potassium chloride (KCl), potassium ferricyanide (K3[Fe(CN)6]), potassium hexacyanoferrate (II) (K4[Fe(CN)6]∙3H2O), metronidazole (MTR, 99%), fluconazole (FLU, 98%), clarithromycin (CLA, 98%), and tetracycline (TC, 98%) were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Sodium phosphate dibasic dodecahydrate (Na2HPO4·12H2O) and Sodium phosphate monobasic dehydrate (NaH2PO4·2H2O) were purchased from Aladdin Chemistry Co., Ltd. (Shanghia, China). All the solutions were prepared with ultrapure water.
5
Antifungal Topical Formulation Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluconazole (Macklin, China), HPMC E5 LV, methyl paraben, propyl paraben, hydrochloric acid, potassium dihydrogen phosphate (Loba Chemie, India), xanthan gum (Titan Biotech, India), polyethylene sorbitan monooleate (tween 80), clove oil (Molychem, India), sorbitan monolaurate (span 20) (Acros Organics, USA), cinnamon leaf oil (Deo Organics, Sri Lanka), palm olein (raffles oil, Nigeria), methanol (BDH, UK), Strat-M™ membrane, 0.45 μm (Millipore, UK), filter units (0.45 μm), Candida albicans (clinical isolate grown at 37 °C for 24 h on Sabouraud dextrose agar), and deionised water.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!