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Antibodies for flow cytometry

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Antibodies for flow cytometry are laboratory reagents used to identify and quantify specific cell surface or intracellular markers in a sample. They are designed to bind to specific target molecules on cells, enabling their detection and analysis through flow cytometry techniques.

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Lab products found in correlation

13 plex cytometric bead arrays cba, by BioLegend (2 mentions) J t baker ultra lc ms grade acetonitrile, by Avantor (2 mentions) 2 2 4 4 d4 cholic acid, by Cambridge Isotopes (2 mentions) 2 2 4 4 d4 deoxycholic acid, by Cambridge Isotopes (2 mentions) 2 2 4 4 d4chenodeoxycholic acid, by Cambridge Isotopes (2 mentions) 2 2 4 4 d4 ursodeoxycholic acid, by Cambridge Isotopes (2 mentions) 2 2 4 4 d4 lithocholic acid, by Cambridge Isotopes (2 mentions) 2 2 4 4 d4 taurocholic acid, by Alsachim (2 mentions) Millipore amicon ultrafree ptfe membranecentrifugal filters 0.2 μm, by Merck Group (2 mentions) Lc ms ultra grade formic acid fluka, by Merck Group (2 mentions) Ficoll paque plus, by GE Healthcare (1 mentions) Fibroblast growth factor (fgf), by Thermo Fisher Scientific (1 mentions) Tgf β1, by Thermo Fisher Scientific (1 mentions) Anti p smad2 3, by Cell Signaling Technology (1 mentions) Anti smad2 3, by Cell Signaling Technology (1 mentions) Anti ifn γ, by R&D Systems (1 mentions) Anti cd3 clone 145 2c11, by BD (1 mentions) Dextran sodium sulphate, by MP Biomedicals (1 mentions) Tgf β, by Thermo Fisher Scientific (1 mentions) Il 17a, by BD (1 mentions)

5 protocols using antibodies for flow cytometry

1

Isolation and Characterization of Immune Cells

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SCID, SCID/Beige, and C57BL/6 mice were purchased from Harlan Laboratories (Madison, WI). Blood collection from healthy donors was approved by the Institutional Review Board. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare Life Sciences, Piscataway, NJ). The parent OVCAR-3 and B16 cell lines were obtained from ATCC (Manassas, VA). Cell culture media was purchased from Mediatech (Manassas, VA) and antibodies for flow cytometry were purchased from BD Biosciences (San Diego, CA) unless noted otherwise.
Felder M., Kapur A., Rakhmilevich A.L., Qu X., Sondel P.M., Gillies S.D., Connor J, & Patankar M.S. (2019). MUC16 suppresses human and murine innate immune responses. Gynecologic oncology, 152(3), 618-628.
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2

Mesenchymal Stem Cell Differentiation

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Dulbecco's Modified Eagle Medium (DMEM) and F12 medium (Sigma, St. Louis, MO, USA), fetal bovine serum (FBS) (Beijing Yuanheng Jinma, China), TGF-β1 (PEPRO Tech, Hamburg, Germany), and fibroblast growth factor (FGF) (PEPRO Tech) were purchased. Agents for histochemical staining, including toluidine blue (Beijing Chemical Reagent Co. Beijing, China), antibodies for flow cytometry (BD Pharmingen, San Diego, CA, USA), PE-conjugated mouse anti-human antibody (BD Pharmingen), and human ribonucleoprotein antibody (Chemicon, Temecula, CA, USA), were purchased.
Liu S., Jia Y., Yuan M., Guo W., Huang J., Zhao B., Peng J., Xu W., Lu S, & Guo Q. (2017). Repair of Osteochondral Defects Using Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells in a Rabbit Model. BioMed Research International, 2017, 8760383.
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3

Immune Regulation Pathway Assessment

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Antibodies for flow cytometry were obtained from BD Biosciences, eBioscience or Biolegend. Anti-CD3 (clone-145-2c11) (Cat.no. 553057) and anti-CD28 (clone-37.1) (Cat.no. 553294) were purchased from BD Biosciences. Anti-pSmad2/3 (Cat. no. 8828) and anti-Smad2/3 (Cat. no. 5678) were purchased from Cell Signaling Technology. Anti- IFN-γ and anti-IL-4 and recombinant cytokines IL-6 and IL-2 were purchased from R&D Systems. TGF-β was purchased from Peprotech. Dextran sodium sulphate (DSS) (Cat. no. 0216011050) was purchased from MP Biomedicals. 2-Deoxyglucose (2-DG) (Cat. no. D6134), Collagenase (Cat. no. C5138), Phorbol 12-myristate 13-acetate (PMA) (Cat. no. P8139), and Ionomycin (Cat. no. I0634) were purchased from sigma. IL-17A and IFN-γ ELISA sets were purchased from BD Biosciences. ELISA kits for Anti-dsDNA and Anti-Nuclear Antibody were purchased from Alpha Diagnostic International.
Kalim K.W., Yang J.Q., Li Y., Meng Y., Zheng Y, & Guo F. (2018). Reciprocal regulation of glycolysis-driven Th17 pathogenicity and Treg stability by Cdc42. Journal of immunology (Baltimore, Md. : 1950), 200(7), 2313-2326.
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4

Comprehensive Multi-Analyte Measurements

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Antibodies for flow-cytometry were purchased from BD biosciences (San
Jose, CA). Cytokine levels were determined using 13-plex Cytometric bead arrays
(CBA) from Biolegend (San Diego, CA). Mass-spectrometry reagents were sourced
from various vendors as follows: J.T. Baker Ultra LC/MS-grade acetonitrile
(ACN), methanol (MeOH), and water were purchased from VWR International (Radnor,
PA). LC/MS Ultra-grade formic acid (Fluka) was purchased from Sigma-Aldrich
(Saint Louis, MO, USA). Internal standards 2,2,4,4-D4 cholic acid,
2,2,4,4-D4 deoxycholic acid, 2,2,4,4-D4chenodeoxycholic acid, 2,2,4,4-D4 ursodeoxycholic acid, and
2,2,4,4-D4 lithocholic acid were purchased from Cambridge Isotope
Laboratories (Andover, MA USA). 2,2,4,4-D4 taurocholic acid was
purchased from AlsaChim (France). Millipore Amicon Ultrafree PTFE membrane
centrifugal filters (0.2 μm), and Millipore Amicon Ultra 0.5 mL 3,000
MWCO centrifugal filters were purchased from Thermo Fisher Scientific (Waltham,
MA USA)
Banerjee S., Sindberg G., Wang F., Meng J., Sharma U., Zhang L., Dauer P., Chen C., Dalluge J., Johnson T, & Roy S. (2016). Opioid-induced gut microbial disruption and bile dysregulation leads to gut barrier compromise and sustained systemic inflammation. Mucosal immunology, 9(6), 1418-1428.
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5

Comprehensive Multi-Analyte Measurements

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies for flow-cytometry were purchased from BD biosciences (San
Jose, CA). Cytokine levels were determined using 13-plex Cytometric bead arrays
(CBA) from Biolegend (San Diego, CA). Mass-spectrometry reagents were sourced
from various vendors as follows: J.T. Baker Ultra LC/MS-grade acetonitrile
(ACN), methanol (MeOH), and water were purchased from VWR International (Radnor,
PA). LC/MS Ultra-grade formic acid (Fluka) was purchased from Sigma-Aldrich
(Saint Louis, MO, USA). Internal standards 2,2,4,4-D4 cholic acid,
2,2,4,4-D4 deoxycholic acid, 2,2,4,4-D4chenodeoxycholic acid, 2,2,4,4-D4 ursodeoxycholic acid, and
2,2,4,4-D4 lithocholic acid were purchased from Cambridge Isotope
Laboratories (Andover, MA USA). 2,2,4,4-D4 taurocholic acid was
purchased from AlsaChim (France). Millipore Amicon Ultrafree PTFE membrane
centrifugal filters (0.2 μm), and Millipore Amicon Ultra 0.5 mL 3,000
MWCO centrifugal filters were purchased from Thermo Fisher Scientific (Waltham,
MA USA)
Banerjee S., Sindberg G., Wang F., Meng J., Sharma U., Zhang L., Dauer P., Chen C., Dalluge J., Johnson T, & Roy S. (2016). Opioid-induced gut microbial disruption and bile dysregulation leads to gut barrier compromise and sustained systemic inflammation. Mucosal immunology, 9(6), 1418-1428.
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