Hrp conjugated anti rabbit igg antibody

Palates of embryos was extracted at E13.5 days. Western blotting was carried out as previously described [21 (link)]. Proteins were transferred to PVDF membranes (Millipore, Billerica, MA, USA), which were incubated overnight at 4 °C with rabbit anti-FGF9 antibody (1:500, ab206408, MA, Abcam), rabbit anti-TCF7L2/TCF4 antibody (1:1000, 13838-1-AP; Proteintech, Wuhan, China), rabbit anti-β-catenin antibody (1:500, ab223075; Abcam), rabbit anti-HAS2 antibody (1:500, ab290669; Abcam), and rabbit anti-GADPH antibody (1:5000, Proteintech, Wuhan, China). Afterwards, these primary antibodies were detected using HRP-conjugated anti-rabbit IgG antibody (Servicebio, Wuhan, China) for 1 h at room temperature. Finally, the protein-specific signals were detected using a Bio-Rad ChemiDoc™ MP Imaging System.

Ipsilateral hemispheres protein homogenate was extracted from mouse brain 3 days after ICH. Western blotting was performed as we previously described [21 (link)]. Proteins were electrophoresed and transferred onto a PVDF membrane (Merck KGaA, Darmstadt, Germany). After being blocked, membranes were incubated overnight at 4 °C with mouse anti-EMMPRIN monoclonal antibody (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-MMP-9 polyclonal antibody (1:1000, Abcam, Cambridge, MA, USA), rabbit anti-ZO-1 polyclonal antibody (1:1000, Proteintech, Wuhan, China), rabbit anti-occludin polyclonal antibody (1:1000, Abcam, Cambridge, MA, USA), and GADPH antibody (1:5000, Proteintech, Wuhan, China). Afterward, membranes were incubated for 1 h at room temperature with the HRP-conjugated anti-rabbit IgG antibody (Servicebio, Wuhan, China) or the anti-mouse IgG antibody (Servicebio, Wuhan, China). Finally, the protein-specific signals were detected using a BIO-RAD ChemiDoc™ MP Imaging System.