The surgical procedures used here were identical to those described previously (Ainge et al., 2007 ). Briefly, animals were anaesthetised using Isoflurane inhalation anaesthetic (Abbott Laboratories) delivered using medical oxygen. Hydration was maintained by administration of 2.5 ml 5% glucose and 1 ml 0.9% saline. Animals were also given anti‐inflammatory analgesia (small animal Carprofen/Rimadyl, Pfizer, UK). In a stereotaxic frame, the electrode tips were lowered to the CA1 coordinates (−3.48 mm AP from bregma, +/−2.4 mm ML from the midline, −1.8 mm DV from dura surface). Skull screws were embedded in the skull and the drive assembly was anchored using dental cement mixed with silver sulfate to a 1% concentration (Ag2SO4, Sigma‐Aldrich, St. Louis). At least one week of recovery time passed before screening and recording took place.
Carprofen rimadyl
Manufactured by Pfizer
Sourced in United Kingdom
Carprofen (Rimadyl) is a non-steroidal anti-inflammatory drug (NSAID) used for the control of pain and inflammation associated with osteoarthritis in dogs. It is available in tablet and injectable formulations for veterinary use.
Automatically generated - may contain errors
Lab products found in correlation
Ag2so4, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Corticosterone, by Merck Group (1 mentions)
Isoflurane aerrane, by Baxter (1 mentions)
Harmaline hydrochloride dihydrate, by Merck Group (1 mentions)
5 meo dmt oxalate, by Merck Group (1 mentions)
Mdl 100907, by Sanofi (1 mentions)
6 protocols using carprofen rimadyl
1
Surgical Implantation of Hippocampal Electrodes
2
Photoconversion of Kaede Transgenic Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Under anaesthesia, the small intestine of Kaede transgenic mice was exposed following laparotomy with a small midline incision. Two- to three-centimetre sections of tissue were exposed to violet light (395 nm UV LED) for 2–3 min each, while PPs and mLN were protected from exposure using high-density black card. Following replacement of the small intestine, the muscle layer was sutured and the skin closed with surgical clips. Zero-hour controls were taken immediately, while mice in 24-h time point were allowed to recover. The small intestine of control mice was exposed to ambient light. Before surgery, the mice received subcutaneous analgesics Carprofen (Rimadyl, Pfizer) and Buprenorphine (Vetergesic, Reckitt Benckiser Healthcare) at 0.1 ml and 0.15 ml per 100 g, respectively.
3
Adrenalectomized Rats for Corticosterone Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male Sprague-Dawley rats (250–300 g) (Harlan, Bicester, UK) were individually caged in soundproof rooms under standard conditions with standard chow and water available ad libitum. All CORT infusions were conducted in rats under 14:10 light/dark cycle (lights on at Zt 0 hr) whilst automated blood sampling experiments were under 12:12 light/dark cycle.
Rats were anaesthetised with a combination of Isoflurane (100% w/w liquid vapour (Merial, UK)) and medical air during bilateral adrenalectomy, jugular vein or/ and carotid artery cannulation. The cannula (Smith Medicals, UK) was exteriorised via craniotomy or a vascular access button and attached to a spring and swivel system (S9 Fig ). Post-operative analgesic 1 mg of Carprofen (Rimadyl, Pfizer, UK), 25 μg dexamethasone (Sigma, UK) and 2.5 ml of glucose (5%)/ saline (0.9%) were administered subcutaneously. For 5 days post-surgical recovery, 0.9% saline drinking water was supplemented with 0.15 μg/L of corticosterone (Sigma, UK) until 16 hours prior to infusion, whereupon 0.9% saline drinking water was provided ad libitum to ensure complete CORT washout from circulation. Implanted cannula was ‘flushed’ daily via withdrawal of blood and replaced with fresh heparinised saline (1:100) to maintain patency.
Rats were anaesthetised with a combination of Isoflurane (100% w/w liquid vapour (Merial, UK)) and medical air during bilateral adrenalectomy, jugular vein or/ and carotid artery cannulation. The cannula (Smith Medicals, UK) was exteriorised via craniotomy or a vascular access button and attached to a spring and swivel system (
4
Pharmacological Agents in Behavioral Study
5
Standardized Anesthesia and Analgesia Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For all surgical procedures, animals were anesthetized with 4% isoflurane until unconscious followed by 2% isoflurane during surgery. All animals received analgesia (Temgesic/Buprenorphine, Schering-Plough, 0.1 mg/kg body weight and Rimadyl/Carprofen, Pfizer, 5 mg/kg body weight; subcutaneous injection) for post-operative pain relief and a uniform layer of eye gel (Viscotears/Carbomer, 2 mg/g, Novartis) was applied onto the eye ball to prevent drying. All animals received local anesthesia (Xylocaine/Lidocaine, AstraZeneca, 10 mg/ml and Marcain/Bupivacaine, AstraZeneca, 2 mg/kg body weight) 2–3 min prior to craniotomies and spinal or brain lesions. For recovery from surgery, animals were placed on a heating pad and only returned to their home cage once they were fully awake.
6
Zoledronate and Vγ9Vδ2 T Cells for Tumor Therapy
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!