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NOSH-Aspirin (NBS-1120) 4-(3-thioxo-3H-1,2-dithiol-5-yl)phenyl 2-((4-(nitrooxy)butanoyl)oxy), benzoate was synthesized as described previously [19 (link)] and was a gift from Avicenna Pharmaceuticals Inc, (New York, NY). The structural components of NOSH-Aspirin are shown in Figure 1. Aspirin was obtained from Sigma (St Louis, MO). Stock solutions (10 mM or 100 mM) were made in DMSO; final DMSO concentration was adjusted in all media to 1%. The pancaspase inhibitor z-VAD-FMK (z-VAD) was obtained from Promega (Madison, WI) and used according to the manufacturer’s instructions. Human pancreatic cancer MIA PaCa-2 (CRL-1420) and BxPC-3 (CRL-1687) cells were purchased from American Type Culture Collection (ATCC, Manassas, VA) with verification of authenticity indicating them to be mycoplasma-free; human normal pancreatic epithelial ACBRI 515 was purchased from the European Collection of Cell Culture (ECACC, Salisbury, UK) and certified as such by the supplier. Cells were maintained in RPMI-1640 media (Invitrogen, Carlsbad, CA) that was supplemented with 10% fetal bovine serum and 100 μg/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA) and maintained at 37°C and in an atmosphere of 5% CO₂/95% air.