Ethanol

Chemicals were obtained from commercial sources. Chromatography material (reverse-Phase high-Performance Liquid Chromatography, (RP-HPLC)), phosphate buffered saline (PBS, pH 7.4), ethanol, acetonitrile, trifluoroacetic acid (TFA), sodium dodecyl sulfate (SDS), acrylamide, ammonium persulfate, N,N,N′,N′-tetramethyl ethylenediamine (TEMED), β-mercaptoethanol and coomassie brilliant blue R-250 were obtained from Bio-Rad (Hercules, CA, USA).
PC, 4-nitro-3-octanoyloxybenzoic acid (4N3OBA), NaCl, CaCl₂, Tris-HCl, bovine serum albumin (BSA), sodium taurodeoxycholate (NaTDC), sodium deoxycholate (NaDC), ethylenediaminetetraacetic acid (EDTA), Triton X₁₀₀, protein markers for molecular mass, and commercial PLA₂ from Naja mossambica mossambica (Nm-PLA₂, Sigma P7778) were purchased from Sigma Aldrich (St. Quentin-Fallavier, France).
Dulbecco’s Modified Eagles Medium, fetal calf serum, glutamine, streptomycin, penicillin, and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) were purchased from Life Technologies, Paisley, UK.

Potassium persulfate (99.5%), sodium carbonate (99%), Trolox (98%), 2,2-azinobis (3-ethylbenothiazoline-6-sulfonic acid) diammonium salt (ABTS) (98%), Folin Ciocalteu’s phenol reagent and Gallic acid (98%) were purchased from Sigma-Aldrich, South Africa. Iron (III) chloride 6-hydrate (97%), dimethyl sulfoxide (DMSO) (99.9%), Iron (II) sulfate 7-hydrate and acetic acid (87%); 2,4,6-Tri(2-pyridyl)-s-triazine (TPTZ) (99%), 2,2-diphenyl-1-picrylhydrazyl (DPPH) (96%), hydrochloric acid (35%), ascorbic acid (99%), sodium chloride (99.9%), and ethanol (99%) were purchased from BioRad, South Africa.