Dead cell apoptosis kit with fitc annexin 5 and pi

Manufactured by BD

Sourced in United States

The Dead Cell Apoptosis Kit with FITC-Annexin V and PI is a laboratory tool used to detect and quantify apoptotic and necrotic cells. It utilizes Annexin V, a calcium-dependent phospholipid-binding protein, and Propidium Iodide (PI), a DNA-binding dye, to differentiate between live, apoptotic, and necrotic cells through flow cytometry analysis.

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Lab products found in correlation

Coulter epics xl flow cytometer, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Annexin V-FITC and PI apoptosis kit Annexin V-FITC and PI kit Annexin V-FITC and PI Annexin V-FITC Apoptosis Kit Annexin-V and PI kit Annexin V-FITC/PI Annexin V and PI Annexin V Apoptosis Kit Annexin V-FITC kit Annexin V/PI

1 protocol using dead cell apoptosis kit with fitc annexin 5 and pi

Apoptosis Analysis of Hep-2 Cells

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Hep-2 cells were cultured in 6-well plates at a concentration of 2×10⁵ cells/well. Following the aforementioned treatments (part of investigation of sequential treatment with PDT followed by CDDP), the Hep-2 cells were collected by performing trypsin detachment, re-suspension in 1× binding buffer from the Dead Cell Apoptosis Kit with FITC-Annexin V and PI (BD Biosciences Pharmingen, San Jose, CA, USA) at a concentration of 1×10⁶ cells/mL and stained using the Dead Cell Apoptosis Kit with FITC-Annexin V and PI in strict accordance following the manufacturer’s protocol. Coulter Epics XL flow cytometer (Beckman Coulter, Fullerton, CA, USA) was used for analysis of the stained cells.

Xue K., Wang Y.N., Zhao X., Zhang H.X., Yu D, & Jin C.S. (2019). Synergistic effect of meta-tetra(hydroxyphenyl)chlorin-based photodynamic therapy followed by cisplatin on malignant Hep-2 cells. OncoTargets and therapy, 12, 5525-5536.

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