Primary antibodies used for western blotting (WB) or immunocytochemistry (ICC) are as follows: mouse anti-FLAG (Sigma-Aldrich F1804, WB 1:3000), mouse anti-G3BP (Abcam ab56574, ICC 1:600), rabbit anti-HA (Abcam ab9110, WB 1:4000, ICC 1:300), mouse anti-tGFP (OriGene TA150041, WB: 1:1000), mouse anti-GAPDH (Santa Cruz Biotechnology sc32233, WB: 1:1000), mouse anti-FUS (Santa Cruz Biotechnology sc47711, WB: 1:1000), mouse anti-PSF (Santa Cruz Biotechnology sc374502, WB: 1:1000), rabbit anti-mono-methyl arginine (Cell Signaling Technology 8015, WB: 1:1000), and rabbit anti-dimethyl arginine, asymmetric (EMD Millipore 07-414, WB: 1:1000). Secondary antibodies used for western blotting or immunocytochemistry are as follows: goat anti-rabbit IgG (H+L)-HRP (Bio-Rad 1706515, WB 1:2000), goat anti-mouse IgG (H+L)-HRP (Bio-Rad 1706516, WB 1:2000), goat anti-rabbit IgG (H+L)-AlexaFluor488 (Jackson ImmunoResearch 111-545-144, ICC 1:200), goat anti-rabbit IgG (H+L)-Cy5 (Jackson ImmunoResearch 111-175-144, ICC 1:200), goat anti-mouse IgG (H+L)-AlexaFluor488 (Jackson ImmunoResearch 115-545-146, ICC 1:200), and goat anti-mouse IgG (H+L)-Cy5 (Jackson ImmunoResearch 115-175-146, ICC 1:200).
Goat anti rabbit igg h l alexafluor488
Manufactured by Jackson ImmunoResearch
Sourced in United States
Goat anti-rabbit IgG (H+L)-AlexaFluor488 is a secondary antibody conjugate that binds to the heavy and light chains of rabbit IgG antibodies. The antibody is labeled with the AlexaFluor488 fluorescent dye, allowing for detection and visualization of target proteins in various immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
F1804, by Abcam (1 mentions)
Goat anti mouse igg h l hrp, by Bio-Rad (1 mentions)
Goat anti mouse igg h l alexafluor488, by Jackson ImmunoResearch (1 mentions)
Goat anti rabbit igg h l hrp, by Bio-Rad (1 mentions)
Mouse anti fus, by Santa Cruz Biotechnology (1 mentions)
Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti ha, by Abcam (1 mentions)
Mouse anti flag, by Merck Group (1 mentions)
Ab8895, by Abcam (1 mentions)
Ab8580, by Abcam (1 mentions)
Ab6002, by Abcam (1 mentions)
Ab10812, by Abcam (1 mentions)
Anti flag coated m2 magnetic beads, by Merck Group (1 mentions)
Ab4729, by Abcam (1 mentions)
Rat α elav, by Developmental Studies Hybridoma Bank (1 mentions)
2 protocols using goat anti rabbit igg h l alexafluor488
1
Antibody Validation for Western Blot and ICC
2
Chromatin Immunoprecipitation Antibody Panel
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The following antibodies were used for ChIP: H3K4me3 (ab8580; Abcam, Cambridge, MA, USA), H3K4me1 (ab8895; Abcam), H3K9ac (ab10812; Abcam), H3K27ac (ab4729; Abcam) and H3K27me3 (ab6002; Abcam). Antibodies used for immunostaining were as follows: rat-α-Elav (from Developmental Studies Hybridoma Bank, Iowa City, IA, USA) and goat anti-rabbit IgG H&L (Alexa Fluor 488; Jackson ImmunoResearch, West Grove, PA, USA). Anti-Flag-coated M2 magnetic beads (Sigma-Aldrich, St. Louis, MO, USA) were used for pull down.
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