LS-174T and HT-29 human colonic epithelial cells were grown in RPMI 1640 medium (Nakarai Tesque, Inc., Kyoto, Japan) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin (Wako) [23] . Human interleukin (IL)− 6 and tumor necrosis factor (TNF)α were purchased from Roche Diagnostics (Indianapolis, IN), and human IL-8, IL-17A, IL-22, hepatocyte growth factor (HGF), basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF) were from Wako. Cells were treated with 20 ng/mL IL-6, 10 nM IL-8, 1 µg/mL IL-17A, 20 ng/mL IL-22, 20 ng/mL TNFα, 50 ng/mL HGF, 10 nM bFGF, and 10 nM EGF for 24 h.
Hepatocyte growth factor (hgf)
Manufactured by Fujifilm
The HGF is a laboratory equipment product manufactured by Fujifilm. It is designed to perform specific functions in a research or analytical setting. The core function of the HGF is to [DESCRIPTION_NOT_AVAILABLE].
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Nacalai Tesque (1 mentions)
Fetal bovine serum (fbs), by Fujifilm (1 mentions)
Basic fibroblast growth factor (bfgf), by Fujifilm (1 mentions)
Tnf α, by Fujifilm (1 mentions)
Penicillin, by Fujifilm (1 mentions)
Streptomycin, by Fujifilm (1 mentions)
Interleukin 6 (il 6), by Fujifilm (1 mentions)
Il 22, by Fujifilm (1 mentions)
2 protocols using hepatocyte growth factor (hgf)
1
Cytokine and Growth Factor Effects on Colonic Epithelial Cells
2
Proliferation of Gingival Fibroblasts by HGF
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The effect of HGF (Fujifilm Wako Pure Chemical Corp.) on the proliferation of human gingival fibroblasts was assessed using the MTT assay. Human gingival fibroblasts were seeded in 96-well plates and incubated for 24 h, after which the medium was changed as follows: 5, 10, 50, or 100 ng/mL HGF (5HGF, 10HGF, 50HGF, 100HGF). After addition of reagents, cell proliferation was assayed by monitoring the absorbance at 450 nm over a period of 7 days using a spectrophotometer.
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