Sw1573

Calu‐6, SW1573, SW480, T84, SK‐LU‐1, DLD‐1, HCT8, NCI‐H2122, NCI‐H1299, and NCI‐H460 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). LoVo, NCI‐H358, LS174T, and Calu‐1 cells were purchased from Cell Bank of Shanghai Institutes for Biological Sciences, Chinese Academy of Science (Shanghai, China). A549, HCC2998, and NCI‐H23 were kindly provided by National Institute of Biological Sciences (Beijing, China). Calu‐6, LS174T, LoVo, SW1573, T84, and SK‐LU‐1 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Gaithersburg, MD, USA). A549, DLD‐1, HCT8, SW480, HCC2998, NCI‐H2122, NCI‐H23, NCI‐H1299, NCI‐H460, and NCI‐H358 cells were maintained in RPMI‐1640 (Gibco). Calu‐1 cells were maintained in McCoy’s 5A medium (Gibco). All growth media were supplemented with 10% FBS (Thermo Scientific, Waltham, MA, USA), 100 units·mL⁻¹ penicillin (Gibco), and 0.1 mg·mL⁻¹ streptomycin (Gibco). Cell lines were reinstated from frozen stocks and passaged no more than thirty times.

Human lung cancer cell lines, Calu-1, Calu-3, NCI-H1299, NCI-H838, LTEP-S, NCI-H1650, NCI-H1975, NCI-H226, NCI-H460, NCI-H520, NCI-H820, PC-9 and SW1573 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, United States of America). Calu-1 cells were cultured in McCoy’s 5a Medium (Gibco), SK-MES-1 and SW1573 cells were cultured in DMEM medium (Gibco) supplemented with 10% FCS, and the other cancer cell lines were maintained in RPMI-1640/1641/1,642 medium (Gibco) supplemented with 10% FCS (Gibco) and 1% penicillin-streptavidin (Gibco). All cells were cultured at 37°C in 5% CO₂.