The number of living cells was measured by trypan blue exclusion using Vi-Cell analyzer (Beckman Coulter). Cell-cycle distributions were performed on ethanol-fixed cells, stained with propidium iodide and analysed by flow cytometry. Flow cytometry data were acquired using CellQuest Pro (Becton Dickinson) software on the FACS LSRII machine (Becton Dickinson) and were analysed using ModfitLT (Verity) software.
Lsrii facs machine
Manufactured by BD
The BD LSRII FACS machine is a flow cytometry instrument designed for cell analysis and sorting. It is capable of detecting and analyzing multiple parameters of individual cells or particles within a sample. The core function of the BD LSRII FACS machine is to provide high-performance flow cytometry capabilities for scientific and research applications.
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Lab products found in correlation
Cellquest pro, by BD (1 mentions)
Vicell analyzer, by Beckman Coulter (1 mentions)
Live dead fixable green dead cell stain, by Thermo Fisher Scientific (1 mentions)
Guava easycyte flow cytometer, by Merck Group (1 mentions)
Fitc annexin 5 apoptosis detection kit 2, by BD (1 mentions)
Propidium iodide, by Thermo Fisher Scientific (1 mentions)
Incyte software, by Merck Group (1 mentions)
5 protocols using lsrii facs machine
1
Cell Viability and Cell Cycle Analysis
2
RON Expression in T47D Cell Lines
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1 × 106 cells were harvested from T47D wildtype and T47D RON KO cell lines and washed in PBS. Cells were blocked and either paraformaldehyde fixed or stained live. Primary antibody staining was performed using 10 µg/ml of purified anti-RON antibodies. Cells were subsequently incubated with goat anti-mouse FITC-conjugated secondary antibodies (Invitrogen). FACS analysis was performed using FACS LSRII machine (Becton Dickinson). FlowJo (RRID:SCR_008520) (Tree Star Inc. USA) software was used for data analysis.
3
ATRA and TCP Induce Cell Differentiation
4
Cell Cycle Analysis of ATRA and TCP
5
Flow Cytometric Analysis of Apoptosis in AML
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FACS analysis of CD11b (MAC-1) expression by AML cell lines and apoptosis analysis of patient samples after drug treatment were performed using an APC-conjugated human CD11b-specific mouse monoclonal antibody at a 1:10 dilution (Cat# 555388, BD Pharmingen) on a BD LSRII FACS machine (Becton Dickinson) with FACS Diva software. For analysis of apoptosis, the Annexin-V FITC apoptosis detection kit II (Cat# 556570, BD Pharmingen) was used according to the manufacturer’s instructions with Propidium Iodide (Cat# R37169, Invitrogen) at 1 μg/mL on Guava EasyCyte flow cytometer with InCyte software (Merck).
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