Breast cancer tissues were cut into serial 5 μm sections and transferred onto electrostatic slides for immunochemical analysis. These specimens were heated at 65 °C for 30 min, deparaffinized in xylene, and rehydrated in a graded ethanol series. Subsequently, endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 min. Next, 5% bovine serum albumin/1× Tris-buffered saline and Tween-20 were used to reduce nonspecific background staining. IHC staining of FOXP3 (mouse, clone 236A/E7, ab20034; Abcam, Cambridge, UK), interleukin (IL)-33 (rabbit, ab207737; Abcam), and transforming growth factor beta 2 (TGFB2) (mouse, clone SD4, ab36495; Abcam) was performed according to the manufacturer’s instructions. The degree of positivity of the markers was graded as low, medium, or high by one pathologist by visual estimation, and by two surgeons according to the H score. H scores <100, 100–199, and ≥200 were graded as low, medium, and high, respectively.
Ab207737
Manufactured by Abcam
Sourced in United Kingdom, United States
Ab207737 is a laboratory product from Abcam. It is a lab equipment item, but a detailed description is not available while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab207737
1
Immunochemical Analysis of Breast Cancer Tissues
2
Western Blot Analysis of Spinal Cord Proteins
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Protein expressions of GAP-43 (8945, 1:1,000; Cell Signaling Technology, Danvers, MA, USA), ST2 (ab25877, 1:2,000; Abcam), IL-33 (ab207737, 1:2,000; Abcam), CD16 (ab109223, 1:2,000; Abcam), and CD206 (sc-58986, 1:1,000; Santa Cruz Biotechnology) were determined in spinal cord homogenates by western blot analysis as previously described [25] . After the protein concentration was measured using bovine serum albumin, the proteins were separated by SDS-PAGE and then transferred onto a polyvinylidene di uoride membrane. Next, the membranes were blocked with 5% nonfat milk for 60 min and incubated with primary antibodies for 12 h at 4° C. Finally, the membranes were incubated with secondary antibody (A32723, A32754, 1:5,000; Invitrogen) for 1 h, and images were acquired (BioSpectrum 515; LabMode, Borehamwood, UK). Image J (National Institutes of Health, Bethesda, MD, USA) was used to visualize the reaction products for quantifying protein expression.
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