Dmem f12 1 1 medium without phenol red

The human osteosarcoma cell line Saos-2 was used for luciferase reporter assays, western blot experiments and 4C-seq assays. It was obtained from the American Type Culture Collection (ATCC^®, Manassas, VA, USA) and grown in Dulbecco’s Modified Eagle Medium (DMEM; Sigma-Aldrich, St Louis, MO, USA), supplemented with 10% Fetal Bovine Serum (FBS; Gibco, Life Technologies, Carlsbad, CA, USA) and 1% penicillin/streptomycin (p/s; Gibco, Life Technologies, Carlsbad, CA, USA), at 37 °C and 5% of CO₂. Human fetal osteoblast 1.19 (hFOB) cell line and human medulla-derived mesenchymal stem cells (MSCs) were used for 4C-seq assays. The hFOB 1.19 cell line was obtained from ATCC (ATCC^®, Manassas, VA, USA) and grown in DMEM: F12 (1:1) medium without phenol red (Gibco, Life Technologies, Carlsbad, CA, USA), supplemented with 10% FBS and 0.3 mg/mL Geneticin (Gibco, Life Technologies, Carlsbad, CA, USA), at 34 °C and 5% of CO₂. MSCs were kindly provided by Dr. José Manuel Quesada Gómez, from Instituto Maimónides de Investigación Biomédica, Hospital Universitario Reina Sofía, Córdoba, Spain [56 (link)]. These cells were grown in alpha-MEM medium (Gibco, Life Technologies, Carlsbad, CA, USA), supplemented with 10% FBS, 1% p/s and 1x Glutamax (Gibco, Life Technologies, Carlsbad, CA, USA), at 37 °C and 5% of CO₂.

The human osteosarcoma cell line Saos‐2 was used for luciferase reporter assays and 4C‐seq assays. It was obtained from the American Type Culture Collection (ATCC HTB‐85) and grown in DMEM (Sigma‐Aldrich, St. Louis, MO, USA), supplemented with 10% FBS (Gibco–Life Technologies, Grand Island, NY, USA) and 1% penicillin/streptomycin (p/s; Gibco–Life Technologies), at 37°C and 5% of CO₂. The human fetal osteoblast 1.19 (hFOB) cell line and human medulla‐derived mesenchymal stem cells (MSCs) were used for 4C‐seq assays. The hFOB 1.19 cell line was obtained from ATCC (ATCC CRL‐11372) and grown in DMEM:F12 (1:1) medium without phenol red (Gibco–Life Technologies), supplemented with 10% FBS and 0.3 mg/mL geneticin (Gibco–Life Technologies) at 34°C and 5% of CO₂. MSCs were kindly provided by Dr. José Manuel Quesada Gómez from Instituto Maimónides de Investigación Biomédica, Hospital Universitario Reina Sofía, Córdoba, Spain.⁽⁴²⁾ These cells were grown in α‐MEM medium (Gibco–Life Technologies), supplemented with 10% FBS, 1% p/s, and 1x glutamax (Gibco–Life Technologies) at 37°C and 5% of CO₂. Human primary osteoblasts (hOBs) used for expression quantitative trait loci (eQTLs) assays are described in Roca‐Ayats and colleagues.⁽⁴³⁾