Anti myc alexafluora 647

DiIC12 was purchased from Life Technology (Eugene, USA). NBD-PE was purchased from Avanti (Alabaster, USA). anti-myc AlexaFluora-647, anti-biotin, and anti-myc were purchased from Cell Signaling Technologies (Danvers, USA). DTT, tris(2-carboxyethyl)phosphine (TCEP), and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were purchased from Research Products International (Mount Prospect, USA); DTT was prepared fresh for every use. Paraformaldehyde (PFA) was purchased as a 16% stock solution from Electron Microscopy Sciences (Hatfield, USA). CaCl 2 , NaCl, CuCl 2 , Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA), anti-myc magnetic beads and propidium iodide were purchased from Fisher Scientific (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted January 29, 2020. ; https://doi.org/10.1101/2020.01.28.923771 doi: bioRxiv preprint (Fair Lawn, USA). 17-Octadecynoic Acid (17-ODYA) and biotin-azide were purchased from Cayman Chemicals (Ann Arbor, USA). HeLa cell lines were acquired from ATCC (Manassas, USA). Primary rat Schwann cells (RSCs) were a generous gift from the lab of Dr. Bruce Carter at Vanderbilt University.