The above periodic subcultural LO2 and HepG2 cells were respectively seeded into 24-well plates in sextuplicate at a starting density of 5×104 cells/well in 500μl medium and cultured for 48h. The culture medium composition and conditions of the cells in each group were the same as the above. ALT and AST leaking in the culture medium were determined using Roche Cobas Automatic Biochemical Analyzer (Basel, Switzerland). The cultured cells were digested, collected and lysed. The intracellular TG in 200μl lysate/well was quantified with commercially available kit (Nanjing Jiancheng Technology Co. Ltd., Nanjing, China) according to the manufacture's instruction. Each experiment was performed in three independent sets.
Cobas automatic biochemical analyzer
Manufactured by Roche
The Cobas Automatic Biochemical Analyzer is a laboratory instrument designed for the automated analysis of various biochemical parameters in biological samples. It is capable of performing a range of tests, including the quantification of metabolites, enzymes, and other analytes, to support clinical decision-making.
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Lab products found in correlation
5 protocols using cobas automatic biochemical analyzer
1
Quantification of Cellular Metabolites
2
Serum Biomarkers and Renal Function
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Blood samples were collected in the morning from each participant's antecubital vein who had fasted for at least 8 h.
Serum creatinine was measured by the alkaline picrate method. The estimated glomerular filtration rate (eGFR) was calculated as follows: eGFR = 175 × Scr−1.234 × age−0.179(if female, × 0.79), where Scr is serum creatinine concentration (in mg/dL) and age in years22 . Fasting glucose was measured by glucose-oxidase method, total cholesterol (TC) by esterase oxidase-peroxidase, triglycerides (TG) by glycerol phosphate oxidase-peroxidase, and high-density lipoprotein cholesterol (HDLC) by direct measurement after phosphotungstate precipitation. LDLC was calculated by the Friedewald formula when plasma triglycerides were ≤ 4.0 mmol/L and otherwise measured directly23 (link). RC was estimated from a standard lipid profile in the fasting state as TC minus LDLC minus HDLC. The above blood indicators were analyzed using the Roche Cobas automatic biochemical analyzer in an accredited central laboratory in the First People's Hospital of Fuyang (Hangzhou, China), and all laboratory equipment has been calibrated to reduce systematic error effectively.
Serum creatinine was measured by the alkaline picrate method. The estimated glomerular filtration rate (eGFR) was calculated as follows: eGFR = 175 × Scr−1.234 × age−0.179(if female, × 0.79), where Scr is serum creatinine concentration (in mg/dL) and age in years22 . Fasting glucose was measured by glucose-oxidase method, total cholesterol (TC) by esterase oxidase-peroxidase, triglycerides (TG) by glycerol phosphate oxidase-peroxidase, and high-density lipoprotein cholesterol (HDLC) by direct measurement after phosphotungstate precipitation. LDLC was calculated by the Friedewald formula when plasma triglycerides were ≤ 4.0 mmol/L and otherwise measured directly23 (link). RC was estimated from a standard lipid profile in the fasting state as TC minus LDLC minus HDLC. The above blood indicators were analyzed using the Roche Cobas automatic biochemical analyzer in an accredited central laboratory in the First People's Hospital of Fuyang (Hangzhou, China), and all laboratory equipment has been calibrated to reduce systematic error effectively.
3
Serum and Liver Lipid Profiling
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Serum concentrations of TG, TC, LDL-C, HDL-C, ALT and AST were determined using Roche Cobas Automatic Biochemical Analyzer. The liver homogenate of 10% w/v was prepared from the fresh liver tissue and saline. The liver TG was quantified with above commercially available kit.
4
Guangzhou Biobank Cohort Study: Comprehensive Health Assessment
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The baseline examination of Guangzhou Biobank Cohort Study (GBCS) was conducted from 2003 to 2008, and the follow-up from 2008 to 2012. Details of GBCS have been reported previously [19 (link)]. Briefly, GBCS is a 3-way collaboration between Guangzhou Number 12 Hospital and the Universities of Hong Kong and Birmingham, UK. Recruitment of participants was from “The Guangzhou Health and Happiness Association for the Respectable Elders” (GHHARE), a community social and welfare organization. GHHARE unofficially aligned with the municipal government. Membership is open to Guangzhou permanent residents aged 50 years or above for a nominal fee of 4 CNY (≈50 US cents) per month. GHHARE included about 7% of Guangzhou residents in this age group, with branches in all 10 districts of Guangzhou, the capital city of Guangdong province in southern China. Within sex and age group, the GBCS participants had fairly similar prevalence of chronic diseases, such as diabetes and hypertension, as a nationally representative sample of urban Chinese [19 (link)]. The baseline and follow-up examination included an interview concerning lifestyle, family and personal medical history and assessment of weight, height, waist circumference, blood pressure, fasting plasma glucose, lipids and inflammatory markers. Plasma glucose and lipids were measured by Roche COBAS automatic biochemical analyzer.
5
Cystatin C Levels and Kidney Function
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For all visits, cystatin C was detected at admission, and SCr was measured at admission and at least the next 3 consecutive days after contrast exposure by using the COBAS automatic biochemical analyzer (Roche Diagnostics, Basel, Switzerland). Biochemical parameters such as serum glucose, fasting lipid profiles, routine blood tests, and glycated hemoglobin were also measured on admission or following 8 to 12 hours of overnight fasting. Data including baseline demographic, comorbidities, clinical risk factors, clinical treatment, angiographic characteristics, and laboratory results were gathered from medical records.
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