Four-week-old Nf1OPG mice were treated with 10 mg/kg NFκB inhibitor (NFκB-IN; Caffeic acid phenethyl ester, Fisher Scientific, 274310), 275 mg/kg PLX3397-containing or control chow pellets (Free Base), 1 mg/ml anti-IL-1β neutralizing antibodies (R&D System 1060-DE-100), anti-IgG2a control antibodies (R&D Systems), or memantine hydrochloride (20 mg/kg) by intraperitoneal injection. Optic nerves were harvested when the mice reached 12 weeks of age, and the percentage of Ki67+ cells and volume measurements determined as previously reported [16 (link)]. At least 5 mice per group were used in all experiments.
Anti igg2a control antibodies
Manufactured by R&D Systems
Anti-IgG2a control antibodies are laboratory reagents used to detect and quantify the presence of IgG2a antibodies in biological samples. They are designed to provide a reliable and consistent control for assays and experiments involving IgG2a detection.
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Lab products found in correlation
2 protocols using anti igg2a control antibodies
1
Optic Nerve Glial Proliferation in Neurofibromatosis
2
Anti-CCL5 and Anti-CCL2 Therapy in Nf1-OPG Mice
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Two hundred and fifty micrograms of anti-Ccl5 (R&D Systems MAB478) or anti-IgG2a control antibodies (R&D Systems) were intraperitoneally injected into Nf1-OPG mice daily from three to six weeks of age (WOA). In addition, seven-week-old Nf1-OPG mice were injected intraperitoneally five days per week for three consecutive weeks with either 2 mg/kg anti-Ccl2 or control IgG antibodies (BioXCell, BE0185, and BE0091, respectively). Mice were analyzed at 12 and 24 WOA. PLX33397 (PLX) treatments of Nf1-OPG mice involved replacing normal chow (AIN-76A rodent diet; Research Diet Inc.) with 275 mg/kg PLX3397-containing chow (Free Base) from four to seven WOA. PLX-treated (n = 7) or control chow (n = 8) mouse optic nerves were collected and analyzed at 24 WOA.
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