Jsm 651ola

The morphological characteristics of the ticks were observed with an Olympus BX51 microscope and an Olympus DP12 digital camera. Ultrastructural observation was performed by SEM with a JEOL JSM-651OLA. The observed features included overall appearance, and anterior and posterior parts. SEM preparations were performed through Integrated Research and Testing Laboratory Gadjah Mada University procedures. Identification was performed based on morphological features using an identification key [13 -16 (link)].

All chemicals used were of analytical grade, and they were used without further purification. All solutions were made using distilled water. Acetosal, ferric chloride (FeCl₃), folin, ibuprofen, potassium iodide (KI), mercury chloride (HgCl₂), sodium hydroxide (NaOH), sodium nitrite (NaNO₂), and paracetamol were purchased from Merck. Sulphuric acid (H₂SO₄) and ethanol 96% were obtained from Emsure. Metampyrone was obtained from Medialabs. Ethyl acetate was obtained from Bratacho Chemistry. Poly(methyl methacrylate) (PMMA) was obtained from Aldrich Chemistry. The characteristic of polymer membrane was observed by Scanning Electron Microscope-Energy Dispersive X-Ray (SEM-EDX) (Jeol JSM-651OLA). The absorbance measurement was recorded by UV-Visible spectrophotometer (Analytic Jena Specord 200).