L ohp

Human CRC cell line SW620 was purchased from the American Tissue Culture Collection (Manassas, VA, USA). Human CRC cell lines HCT116 and SW480 were purchased from DS Pharmabiomedical (Osaka, Japan). L-OHP-resistant SW620 (SW620-OxR) cells were prepared as previously reported [9 (link)]. SW620, SW620-OxR, and SW480 cells were grown in Leibovitz's L-15 medium (Life Technologies Corp., Carlsbad, CA, USA) plus 10% fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in 100% air. SW620-OxR cells were grown with 80 μM L-OHP (Tokyo Chemical Industry Co., Tokyo, Japan) and were cultured without L-OHP for 1 passage before experiments. HCT116 cells were grown in McCoy's 5A medium (Life Technologies Corp.) plus 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in 5% CO₂ air.