Twenty micrograms of protein was loaded for SDS-PAGE and transferred onto a PVDF membrane for Western blots. Primary antibodies (α-FLAG; Sigma-Aldrich, F1804; α-MSK1; Abcam, ab99412; α-MSK1S376ph; Abcam, ab32190; α-MSK1S212ph; Abcam, ab79499) were used at a 1:1000 dilution in 1X Tris Buffered Saline with 1% Casein (Bio-Rad, 1610782EDU). Secondary α-mouse HRP (Sigma-Aldrich, A6154) or α-rabbit HRP (Abcam, ab6721) were used at a 1:3000 dilution in 1X Tris Buffered Saline with 1% Casein (Bio-Rad, 1610782EDU). Membranes were exposed after addition of ECL (Bio-Rad, 170-5060). Tubulin was detected with hFAB™ Rhodamine Anti-Tubulin Primary Antibody (Bio-Rad, 12004166; 1:3000 dilution).
Hfab rhodamine anti tubulin primary antibody
Manufactured by Bio-Rad
The HFAB™ Rhodamine Anti-Tubulin Primary Antibody is a laboratory reagent used to detect and visualize tubulin, a key structural protein found in the cytoskeleton of eukaryotic cells. The antibody is conjugated with the fluorescent dye rhodamine, allowing for the localization and quantification of tubulin in fixed and permeabilized cells using fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemi luminescence (ecl), by Bio-Rad (2 mentions)
Ab6721, by Abcam (1 mentions)
Ab99412, by Abcam (1 mentions)
α flag, by Merck Group (1 mentions)
Casein, by Bio-Rad (1 mentions)
F1804, by Abcam (1 mentions)
A6154, by Abcam (1 mentions)
Alexa fluor 488 conjugated anti rabbit, by Thermo Fisher Scientific (1 mentions)
Ab5603, by Merck Group (1 mentions)
Ab157524, by Abcam (1 mentions)
Ab6046, by Abcam (1 mentions)
Ab76541, by Abcam (1 mentions)
Alexafluor 647 conjugated anti mouse, by Thermo Fisher Scientific (1 mentions)
3 protocols using hfab rhodamine anti tubulin primary antibody
1
Western Blot Analysis of Phosphorylated MSK1
2
Western Blot Analysis of STAT1 and pSTAT1
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MSCs were lysed in RNA immunoprecipitation (RIPA) buffer. Total protein concentrations were quantified using a BCA assay. 25μg of protein was loaded for SDS-PAGE and transferred onto a PVDF membrane for western blot. Primary antibodies for STAT1 (66545-1, Proteintech, Rosemont, IL) and pSTAT1 (MA5–15071, Thermo, Waltham, MA) were used at a 1:5000 and 1:1000 dilution, respectively, in 1X Tris Buffered Saline with 1% Casein (Bio-Rad, Hercules, CA). Secondary α-mouse HRP (A6154, Sigma-Aldrich, St. Louis, MO) or α-rabbit HRP (ab6721, Abcam, Cambridge, UK) were used at a 1:3000 dilution in 1X Tris Buffered Saline with 1% Casein. Membranes were exposed after addition of ECL (170–5060, Bio-Rad, Hercules, CA). Tubulin was detected with hFAB™ Rhodamine Anti-Tubulin Primary Antibody (12004166, Bio-Rad, Hercules, CA) at 1:3000 dilution.
3
Embryonic Protein Expression Analysis
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Antibodies used for analysis of embryos include rabbit anti–β-tubulin (Abcam, ab6046, polyclonal, lot: GR31927-5), mouse anti–DICER-1 (Santa Cruz Biotechnology, sc-136981, A-2, lot: I1817), mouse anti–CDX-2 (Abcam, ab157524, CDX2-88, lot: GR300552-6), anti-rabbit anti-CDX2 antibody (Abcam, ab76541, lot #GR300552-18), rabbit anti-SOX-2 (EMD Millipore, AB5603, polyclonal, lot: NG1863962 and 3254559), goat anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich, SAB2500450, polyclonal, lot: 6377C2), rabbit anti-GADD45a (Invitrogen, MA5-17014, D.81.E, lot: R12274975), Alexa Fluor 647 anti-mouse/human CD324 (E-cadherin) antibody (BioLegend catalog no. 147307, lot no. B254794), Alexa Fluor 594 FGFR-1 antibody (Santa Cruz Biotechnology, catalog no. sc-57132 AF594, lot no. J0218), Rhodamine anti–β-actin immunoglobulin G (Bio-Rad, 12004163, lot: 64219909), hFAB Rhodamine anti-tubulin primary antibody (Bio-Rad, 12004165, lot no. 64210670). Donkey secondary antibodies Alexa Fluor 647–conjugated anti-mouse (A31571, lot #1903516), Alexa Fluor 594–conjugated anti-mouse (A21203, lot #2066086), and Alexa Fluor 488-conjugated anti-rabbit (A21206) were purchased from Thermo Fisher Scientific, CA, USA. All bands migrated to approximate molecular masses stated by manufacturers or previously published studies (table S2).
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