2-Cl-trityl chloride resin (loading: 0.939 mmol/g), fmoc-amino acids and o-benzotriazol-1-yl-N, N, N', N'-tetramethyluronium hexafluorophosphate (HBTU) were bought from GL Biochem (Shanghai). 4-Chloro-7-nitrobenzol-2-oxa-1, 3-diazole (NBD-Cl) and telmisartan were purchased from Sigma-Aldrich (USA). Cy5.5 NHS ester was obtained from APExBIO (USA). Recombinant MAS1 Protein (2 µg) was obtained from Abnova (China). Fetal bovine serum (FBS), Dulbecco's modified Eagle's medium (DMEM), trypsin, and Penicillin-streptomycin were obtained from Thermo Fisher (USA). DHE fluorescence probe, Apoptosis Analysis Kit, DAPI, and Hoechst 33342 dye were purchased from Molecular Probes (USA). CCK-8 kit was purchased from Dojindo Co. Ltd (Japan). The primary antibodies of anti-AT1R, anti-α-SMA, anti-Bax, anti-Bcl-2, anti-Histone 3, anti-Collagen I, anti-TGF-β1 and anti-cTnT were obtained from Abcam (Britain). The antibodies of anti-AKT, anti-p-AKT, anti-P65, anti-Caspase3 were purchased from Cell Signaling Technology (USA). ELISA kit for IL-6, TNF-α, CRE, ALT, TGF-β1 were bought from MSKBIO (China). All other solvents and reagents were commercially available and used without further purification, unless noted otherwise.
Cy5.5 nhs ester
Manufactured by Apexbio
Sourced in United States
Cy5.5 NHS ester is a fluorescent labeling reagent used for the covalent modification of biomolecules such as proteins, peptides, and nucleic acids. It has an absorption maximum at 673 nm and an emission maximum at 707 nm, making it suitable for applications that require far-red or near-infrared fluorescence detection.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Anti collagen 1, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Telmisartan, by Merck Group (1 mentions)
Anti bax, by Abcam (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti p65, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti at1r, by Abcam (1 mentions)
2 cl trityl chloride resin, by GL Biochem (1 mentions)
Anti histone 3, by Abcam (1 mentions)
Anti bcl 2, by Abcam (1 mentions)
Anti α sma, by Abcam (1 mentions)
Hoechst 33342 dye, by Thermo Fisher Scientific (1 mentions)
Fmoc amino acids, by GL Biochem (1 mentions)
O benzotriazol 1 yl n n n n tetramethyluronium hexafluorophosphate hbtu, by GL Biochem (1 mentions)
Anti ctnt, by Abcam (1 mentions)
Anti caspase 3, by Cell Signaling Technology (1 mentions)
Anti tgf β1, by Abcam (1 mentions)
Matrigel, by Corning (1 mentions)
2 protocols using cy5.5 nhs ester
1
Synthesis and Characterization of Telmisartan-Labeled Probes
2
Fluorescent Peptide Synthesis and Evaluation
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Amino acids, 2-(naphthalene-2-yl) acetic acid, and O-(benzotriazole-1-yl)-N,N,N,N-tetramethyluronium hexafluorophosphate (HBTU) were purchased from Bidepharm (Shanghai, China). 1-Pyrenemethylamine hydrochloride and trifluoroacetic acid (TFA) were obtained from Energy Chemical (Beijing, China). 2-Cl-Trityl chloride resin was bought from Nankai Resin Co. Ltd (Tianjin, China). N,N-Diisopropylethylamine (DIEA) was purchased from J & K (Beijing, China). Glycine, phenylalanine, tyrosine, and methotrexate were bought from Bidepharm (Shanghai, China). Cy5.5 NHS ester was obtained from APExBIO (USA). ELISA kit for IL-6 was bought from MSKBIO (China). Matrigel was bought from Corning (USA). Tubulin, iNOS, IL-6, and TNF-α antibodies used in western blot were purchased from Abcam (ab7291, ab178945, ab259341, and ab215188, respectively). In immunofluorescence staining, F4/80 was obtained from Bioss (bsm-34028 M), iNOs was obtained from Proteintech (18985-1-AP), CD206 was obtained from Abcam (ab64693), CD31 was brought from Servicebio (GB11063-2). DAPI was obtained from Beyotime Biotechnology (Shanghai, China). Commercially available reagents were used without further purification.
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