BVP was purchased from Shanghai Ronghe Pharmaceutical Technology Development Co., Ltd. (Shanghai, China; purity ≥98%), and dissolved in dimethylsulfoxide (DMSO; the final DMSO concentration was <0.1%). Rabbit antihuman polyclonal antibodies recognizing BCL2-associated X, apoptosis regulator (BAX) and B-cell CLL/lymphoma 2 (BCL2) were bought from Abcam (Cambridge, MA, USA). The ROS scavenger N-acetylcysteine (NAC) was purchased from Selleck (Houston, TX, USA). ROS detection kit (S0033S) and Annexin V-FITC apoptosis detection kit (C1062) were bought from Beyotime. Human IGFBP-4 ELISA kit (SEKH-0215) was purchased from Solarbio.
Annexin 5 fitc apoptosis detection kit c1062
Manufactured by Beyotime
Sourced in China
The Annexin V-FITC Apoptosis Detection Kit (C1062) is a laboratory product that is used to detect and quantify apoptosis, a type of programmed cell death. The kit utilizes Annexin V, a calcium-dependent phospholipid-binding protein, which has a high affinity for phosphatidylserine (PS). During apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, exposing it to the external cellular environment. The kit contains Annexin V conjugated with the fluorescent dye FITC, allowing for the detection of apoptotic cells by flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Nf κb p65 sc 372, by Santa Cruz Biotechnology (2 mentions)
Nrf2 c 20 sc 722, by Santa Cruz Biotechnology (2 mentions)
Corm 2 288144, by Merck Group (2 mentions)
N acetyl cysteine a7250 10g, by Merck Group (2 mentions)
Ferrous chloride fecl 2, by Sinopharm (2 mentions)
Diamidino 2 phenylindole dihydrochloride dapi c10005, by Beyotime (2 mentions)
Reactive oxygen species assay kit s0033, by Beyotime (2 mentions)
Anti nqo1 antibody ab2346, by Abcam (2 mentions)
Corm 2, by Merck Group (2 mentions)
N acetylcysteine nac, by Selleck Chemicals (1 mentions)
Ros detection kit s0033s, by Beyotime (1 mentions)
Sod a001 3 2, by Nanjing Jiancheng (1 mentions)
Gsh a006 2 1, by Nanjing Jiancheng (1 mentions)
Ros assay kit s0033, by Beyotime (1 mentions)
Hrp conjugated affinipure goat anti rabbit igg h l, by Proteintech (1 mentions)
Bca protein assay kit p0012, by Beyotime (1 mentions)
Ab137550, by Abcam (1 mentions)
Sa00001 2, by Proteintech (1 mentions)
A003 4 1, by Nanjing Jiancheng (1 mentions)
A015 2 1, by Nanjing Jiancheng (1 mentions)
5 protocols using annexin 5 fitc apoptosis detection kit c1062
1
Exploring Apoptosis Regulation with BVP
2
CORM-2 and NAC Modulate Stem Cell Behavior
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CORM-2 (288144) and N-acetyl-cysteine (NAC) (A7250-10G) were purchased from Sigma-Aldrich (St.
Louis, MO, USA).CORM-2 was dissolved in dimethyl sulfoxide, and then diluted in culture media to achieve the required concentrations. Inactivated CORM-2 (iCORM-2) was prepared by dissolving CORM-2 under the same conditions for 3 days at room temperature to liberate all CO from the molecule [14]. NSC C17.2, a stable, fully characterized, mouse stem cell line [15] , was kindly provided by Prof. Jin WL (Institute of Nano Biomedicine and Engineering, Shanghai Jiao Tong University, Shanghai, China). Ferrous chloride (FeCl 2 ) was purchased from Sinopharm Chemical Reagent (Shanghai, China). Annexin V-FITC Apoptosis Detection Kit (C1062), Diamidino-2-phenylindole dihydrochloride (DAPI) (C10005) and Reactive Oxygen Species Assay Kit (S0033) were from Beyotime Biotechnology (Shanghai, China). The primary antibodies Nrf2 (C-20) (SC-722) and NF-κB P65 (SC-372) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Anti-NQO1 antibody (ab2346) was from Abcam (Cambridge, UK). ß-Actin (4970),Tublin(2148) and H3 (12167), were from Cell Signaling Technology (USA).
Louis, MO, USA).CORM-2 was dissolved in dimethyl sulfoxide, and then diluted in culture media to achieve the required concentrations. Inactivated CORM-2 (iCORM-2) was prepared by dissolving CORM-2 under the same conditions for 3 days at room temperature to liberate all CO from the molecule [14]. NSC C17.2, a stable, fully characterized, mouse stem cell line [15] , was kindly provided by Prof. Jin WL (Institute of Nano Biomedicine and Engineering, Shanghai Jiao Tong University, Shanghai, China). Ferrous chloride (FeCl 2 ) was purchased from Sinopharm Chemical Reagent (Shanghai, China). Annexin V-FITC Apoptosis Detection Kit (C1062), Diamidino-2-phenylindole dihydrochloride (DAPI) (C10005) and Reactive Oxygen Species Assay Kit (S0033) were from Beyotime Biotechnology (Shanghai, China). The primary antibodies Nrf2 (C-20) (SC-722) and NF-κB P65 (SC-372) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Anti-NQO1 antibody (ab2346) was from Abcam (Cambridge, UK). ß-Actin (4970),Tublin(2148) and H3 (12167), were from Cell Signaling Technology (USA).
3
CORM-2 and NAC Modulate Stem Cell Behavior
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CORM-2 (288144) and N-acetyl-cysteine (NAC) (A7250-10G) were purchased from Sigma-Aldrich (St.
Louis, MO, USA).CORM-2 was dissolved in dimethyl sulfoxide, and then diluted in culture media to achieve the required concentrations. Inactivated CORM-2 (iCORM-2) was prepared by dissolving CORM-2 under the same conditions for 3 days at room temperature to liberate all CO from the molecule [14]. NSC C17.2, a stable, fully characterized, mouse stem cell line [15] , was kindly provided by Prof. Jin WL (Institute of Nano Biomedicine and Engineering, Shanghai Jiao Tong University, Shanghai, China). Ferrous chloride (FeCl 2 ) was purchased from Sinopharm Chemical Reagent (Shanghai, China). Annexin V-FITC Apoptosis Detection Kit (C1062), Diamidino-2-phenylindole dihydrochloride (DAPI) (C10005) and Reactive Oxygen Species Assay Kit (S0033) were from Beyotime Biotechnology (Shanghai, China). The primary antibodies Nrf2 (C-20) (SC-722) and NF-κB P65 (SC-372) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Anti-NQO1 antibody (ab2346) was from Abcam (Cambridge, UK). ß-Actin (4970),Tublin(2148) and H3 (12167), were from Cell Signaling Technology (USA).
Louis, MO, USA).CORM-2 was dissolved in dimethyl sulfoxide, and then diluted in culture media to achieve the required concentrations. Inactivated CORM-2 (iCORM-2) was prepared by dissolving CORM-2 under the same conditions for 3 days at room temperature to liberate all CO from the molecule [14]. NSC C17.2, a stable, fully characterized, mouse stem cell line [15] , was kindly provided by Prof. Jin WL (Institute of Nano Biomedicine and Engineering, Shanghai Jiao Tong University, Shanghai, China). Ferrous chloride (FeCl 2 ) was purchased from Sinopharm Chemical Reagent (Shanghai, China). Annexin V-FITC Apoptosis Detection Kit (C1062), Diamidino-2-phenylindole dihydrochloride (DAPI) (C10005) and Reactive Oxygen Species Assay Kit (S0033) were from Beyotime Biotechnology (Shanghai, China). The primary antibodies Nrf2 (C-20) (SC-722) and NF-κB P65 (SC-372) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Anti-NQO1 antibody (ab2346) was from Abcam (Cambridge, UK). ß-Actin (4970),Tublin(2148) and H3 (12167), were from Cell Signaling Technology (USA).
4
Zearalenone-induced Oxidative Stress Attenuation
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Zearalenone (ZEA, Z2125) and lipopolysaccharide (LPS, SMB00610) were obtained from Sigma (St Louis, Missouri, USA). Quercetin (B20527, purity ≥ 98%) was purchased from Shanghai Yuanye (Shanghai, China). DMEM/F12 (SH30069.03), fetal bovine serum (FBS, SH30406.05), and penicillin-streptomycin (SV30010) were provided by HyClone (Logan, UT, USA). ROS assay kit (S0033S), BCA protein assay kit (P0012), and Annexin V-FITC Apoptosis Detection Kit (C1062S) were purchased from Beyotime Biotechnology (Shanghai, China). Assay kits for superoxide dismutase (SOD, A001-3-2), lactate dehydrogenase (LDH, A020-2-2), total antioxidant capacity (T-AOC, A015-2-1), glutathione (GSH, A006-2-1), and malondialdehyde (MDA, A003-4-1) were purchased from Nanjing Jiancheng (Nanjing, China). The primary antibody against Nrf2 (1:1000, ab137550) as purchased from Abcam (Cambridge, MA, USA). The primary antibodies against HO-1 (1:1000, 10701-1-AP), NQO1 (1:1000, 11451-1-AP), ZO-1 (1:5000, 21773-1-AP), Occludin (1:5000, 27260-1-AP), Claudin 1 (1:1000, 28674-1-AP), β-actin (1:2000, 20536-1-AP), and HRP-conjugated Affinipure Goat AntiRabbit IgG (H+L) (1:4000, SA00001-2) were purchased from Proteintech (Wuhan, China). TRIzol (Cat No. 15596026) and PVDF membranes (Cat No. 24585) were purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA).
5
Annexin V-FITC Apoptosis Assay
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Cells apoptosis were analyzed using the Annexin V-FITC Apoptosis Detection Kit; C1062S; Beyotime Biotechnology, Shanghai, China by a Beckman CytoFLEX FCM Flow Cytometer (Brea, CA, USA). The green fluorescence of Annexin V-FITC was detected using the FITC channel (FL1), and the red fluorescence of PI was detected using the PI channel (FL2). The parameters of flow cytometry were as follows: excitation wavelength Ex = 488 nm, emission wavelength FL1 (Em = 525 ± 20 nm) and FL2 (Em = 585 ± 21 nm). FlowJo software was used to analyze the proportion and number of different cell states (mainly early apoptosis, late apoptosis, and death).
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