Twenty-four-hour urine was collected from knockin (Col4a3 mut/mut), compound heterozygous (Col4a3 mut/−) and wild-type (WT) mice (Col4a3+/+) every 4-weeks over 14 months. Urine collected in metabolic cages for 24-h, was evaluated for proteinuria and hematuria. Protein was tested qualitatively, by microelectrophoresis of 20 μL urine on SDS-polyacrylamide gel and Coomassie Blue staining followed by densitometry, using bovine serum albumin as molecular-weight standard. Hematuria was tested by using a dipstick test (Roche) and by calculation of red blood cell number/ml urine, under optic microscopy. Serum parameters (urea and creatinine) were analyzed in blood collected from the heart of sacrificed animals.
Dipstick test
Manufactured by Roche
The Dipstick test is a rapid, qualitative diagnostic tool used for the screening and detection of various analytes in a sample, such as urine or other bodily fluids. It consists of a small, disposable test strip impregnated with chemical reagents that change color when exposed to the target analyte, providing a visual result.
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Lab products found in correlation
2 protocols using dipstick test
1
Murine Kidney Disease Biomarkers
2
Streptozotocin-Induced Rat Diabetes Model
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Streptozotocin destroys pancreatic beta cells and is used in experimental models of type 1 diabetes. 19 In this study, a single intraperitoneal streptozotocin injection (60 mg/kg, prepared in 0.1 mol/L citrate buffer, pH 4.5) was used as described. 16 The state of diabetes was confirmed by glucosuria using dipstick urine test (Combur, Roche Diagnostics, France) 1 week after the injection. Urine test was repeated once a week during the study for detection of glucosuria. Proteinuria was detected using dipstick test (Roche). In addition, blood glucose levels were measured at the end of the study in truncal blood collected immediately after decapitation using a commercial kit (Gluco-quant Glucose/HK, Roche Diagnostics). Rats without sustained diabetes indicated by glucosuria at least 20 mmol/L 14 days after the injection till the end of the study were excluded from the study.
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