Analytical-grade chloroform, methanol, petroleum ether, and ethyl acetate (Loba Chemie Pvt. Ltd., Mumbai, India) were used for extraction and column elution. A rotary evaporator (Labo Rota 4000, Heidolph Instrument) was used for extract concentration. Column chromatographic separation was done on silica gel 60–120 mm (Merck, Darmstadt, Germany). Further purification was carried out on Sephadex LH-20. TLC analysis was performed on pre-coated silica gel 60 F254 plates (Merck, Darmstadt, Germany) using petroleum ether and ethyl acetate as mobile phase. Visualization of spots on the TLC plate was achieved by using UV lamps (UV-Tec 254 nm and 365 nm) and iodine vapor. 1H NMR (400 MHz and 500 MHz) and 13C NMR (100 MHz and 125 MHz) spectra were recorded on the Bruker Avance 400 and 500 Spectrometers, respectively, using CDCl3 as a solvent. Distilled water, Mueller-Hinton agar, nutrient broth, DMSO, gentamicin, and clotrimazole were used for antibacterial and antifungal activity tests.
500 spectrometers
Manufactured by Bruker
The Bruker 500 Spectrometers are high-performance nuclear magnetic resonance (NMR) systems designed for advanced analytical applications. These spectrometers provide precise and reliable measurements of molecular structure and dynamics across a wide range of sample types and research fields.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Loba Chemie (1 mentions)
Petroleum ether, by Merck Group (1 mentions)
Avance 400, by Bruker (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Silica gel 60 f254 plate, by Merck Group (1 mentions)
Ethyl acetate, by Loba Chemie (1 mentions)
Petroleum ether, by Loba Chemie (1 mentions)
6130 quadrupole lc ms, by Agilent Technologies (1 mentions)
6520 accurate mass q tof lc ms, by Agilent Technologies (1 mentions)
2 protocols using 500 spectrometers
1
Phytochemical Extraction and Purification
2
Spectroscopic Characterization and Purification
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1H and 13C
NMR spectra were obtained on Bruker 500 spectrometers with TMS or
residual solvent as standard. All column chromatography was performed
using Dynamic Adsorbents 230–400 mesh silica gel (SiO2) with the indicated solvent system unless otherwise noted. TLC analysis
was performed using 254 nm glass-backed plates and visualized using
UV light (254 nm), and low-resolution mass spectra and purity data
were obtained using an Agilent Technologies 6130 Quadrupole LC/MS.
The purity of the compounds were assessed by analytical HPLC, with
the solvent gradient started at 0% methanol in water and ended at
100% methanol after 8 min with 0.1% of TFA. The purity of all final
tested compounds was determined to be >95% (UV, λ = 254 nm).
High-resolution mass spectrum data were collected on an Agilent 6520
Accurate-Mass Q-TOF LC/MS. HPLC purification was carried out on a
Waters Delta 600 equipped with a Sunfire Prep C18 OBD column (30 mm
× 150 mm, 5 μm) with methanol–water (both containing
0.1% TFA) as mobile phase. The preparative HPLC gradient started at
50% methanol in water and ended at 100% methanol after 40 min with
0.1% of TFA.
NMR spectra were obtained on Bruker 500 spectrometers with TMS or
residual solvent as standard. All column chromatography was performed
using Dynamic Adsorbents 230–400 mesh silica gel (SiO2) with the indicated solvent system unless otherwise noted. TLC analysis
was performed using 254 nm glass-backed plates and visualized using
UV light (254 nm), and low-resolution mass spectra and purity data
were obtained using an Agilent Technologies 6130 Quadrupole LC/MS.
The purity of the compounds were assessed by analytical HPLC, with
the solvent gradient started at 0% methanol in water and ended at
100% methanol after 8 min with 0.1% of TFA. The purity of all final
tested compounds was determined to be >95% (UV, λ = 254 nm).
High-resolution mass spectrum data were collected on an Agilent 6520
Accurate-Mass Q-TOF LC/MS. HPLC purification was carried out on a
Waters Delta 600 equipped with a Sunfire Prep C18 OBD column (30 mm
× 150 mm, 5 μm) with methanol–water (both containing
0.1% TFA) as mobile phase. The preparative HPLC gradient started at
50% methanol in water and ended at 100% methanol after 40 min with
0.1% of TFA.
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