Ab32530

All tissues were fixed in a 10% formaldehyde solution, then embedded in paraffin, and sliced into 5-μm sections. The tissue slides were deparaffinized, rehydrated, and then retrieved with citric acid buffer (pH 6.0, 10 mM) using a standard microwave-based method. Thereafter, the sections were washed and incubated with a blocking solution for 1 h at room temperature after treated with 3% hydrogen peroxide for 10 min. Afterward, the sections were incubated with diluted primary antibodies at 4 °C overnight, followed by washing and incubation with a horseradish peroxidase (HRP)-conjugated secondary antibody. After 30 min at 37 °C, a 3,3′-diaminobenzidine solution was added for color development, and then the sections were counterstained with hematoxylin, dehydrated, and mounted. The results of staining were assessed by two independent pathologists.
All primary antibodies were purchased from Abcam (Cambridge, MA, USA), including anti-UBA2 (ab185955, 1:100), anti-mutant p53 (ab32049, 1:50), anti-c-Myc (ab32072, 1:100), anti-Bax (ab32530, 1:200), anti-cleaved caspase-3 (ab32042, 1:300), and anti-cleaved caspase-9 (ab2324, 1:100).