Incucyte

Manufactured by Merck Group

The IncuCyte is an automated live-cell analysis system that enables real-time monitoring and quantification of cellular processes. It provides continuous, non-invasive imaging and analysis of cells within a standard cell culture incubator.

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Lab products found in correlation

Mitosox red, by Thermo Fisher Scientific (1 mentions) Valinomycin, by Merck Group (1 mentions) Gemcitabine hydrochloride, by Merck Group (1 mentions) Incucyte, by Sartorius (1 mentions) Annexin 5 green reagent, by Sartorius (1 mentions)

2 protocols using incucyte

Mitochondrial Dysfunction in LRRK2 PD Fibroblasts

Cited 1 time

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Previously described (Smith et al., 2016 (link), Korecka & Thomas et al., 2019) deidentified healthy and LRRK2 G2019S patient fibroblasts were obtained from the Coriell and the NIA Aging Cell biorepositories or the Mayo clinic. Conducting research with patient fibroblasts was not considered to be human subject research as it does not involve intervention or interaction with the individual and the samples do not contain identifiable private information. The average age of the control and the mutant fibroblasts was matched to 66 and 67 years, respectively. Mitochondrial super oxide (MitoSOX^™ Red, ThermoFisher Scientific, M36008) levels, induced by 24-hour treatment with the mitochondrial depolarization agent valinomycin (10μM, SigmaAldrich, V0627), were measured in human fibroblasts obtained from healthy individuals (N=4) and PD patients carrying a LRRK2 G2019S mutation (N=5), in the presence of sepiapterin or SPRi3 (pretreatment for 24-hours before addition of valinomycin), using the live cell imaging system IncuCyte.

Cronin S.J., Yu W., Hale A., Licht-Mayer S., Crabtree M.J., Korecka J.A., Tretiakov E.O., Sealey-Cardona M., Somlyay M., Onji M., An M., Fox J.D., Turnes B.L., Gomez-Diaz C., da Luz Scheffer D., Cikes D., Nagy V., Weidinger A., Wolf A., Reither H., Chabloz A., Kavirayani A., Rao S., Andrews N., Latremoliere A., Costigan M., Douglas G., Freitas F.C., Pifl C., Walz R., Konrat R., Mahad D.J., Koslov A.V., Latini A., Isacson O., Harkany T., Hallett P.J., Bagby S., Woolf C.J., Channon K.M., Je H.S, & Penninger J.M. (2023). Crucial neuroprotective roles of the metabolite BH4 in dopaminergic neurons. bioRxiv.

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Organoid Viability Assay with Gemcitabine

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Relative viability of organoids was determined by measuring Annexin V fluorescence in real-time with Incucyte (Essen Bioscience). Organoids were seeded (3000 cells/well) into a U-bottom ULA 96 well-plate (Costar, 7007). Cells were incubated with Annexin V Green Reagent (Essen Bioscience, 4642) and treated with Gemcitabine Hydrochloride (G6423, Sigma) at 3nM, 10nM. and 30nM. Real-time fluorescence was measured with Incucyte during 72 hours.

Calvo I.R., Weber C., Ray M., Brown M., Kirby K., Nandi R.K., Long T.M., Sparrow S.M., Ugolkov A., Qiang W., Zhang Y., Brunetti T., Kindler H., Segal J.P., Rzhetsky A., Mazar A.P., Buschmann M.M., Weichselbaum R., Roggin K, & White K.P. (2018). Human Organoids Share Structural and Genetic Features with Primary Pancreatic Adenocarcinoma Tumors. Molecular cancer research : MCR, 17(1), 70-83.

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