The largest database of trusted experimental protocols

Anti la ab

Manufactured by Abcam

Anti-La Ab is a laboratory product used for the detection and measurement of anti-La antibodies. Anti-La antibodies are autoantibodies that target the La/SSB protein, which is involved in RNA processing. This product can be used in various immunoassay techniques to aid in the diagnosis and monitoring of autoimmune disorders, such as Sjögren's syndrome and systemic lupus erythematosus.

Automatically generated - may contain errors

2 protocols using anti la ab

1

Quantifying Autoantibodies in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-MTg autoantibodies were determined by ELISA, as described in detail previously (36 (link)). Sera from individual mice were diluted 1/50 or 1/100 and plated in duplicate on MTg-coated ELISA plates. Anti-Ro and anti-La Abs were quantified by ELISA. Briefly, plates were coated overnight with 5 μg/ml anti-Ro Ab (Sigma-Aldrich, St. Louis, MO) or anti-La Ab (Abcam, Cambridge, MA) diluted in PBS. After washing with PBS with 0.5% Tween and blocking with 0.2% BSA in PBS, serum was diluted and incubated on plates overnight. Bound Ig was detected with goat anti-mouse IgG–alkaline phosphatase (0.2 μg/ml; Southern-Biotech, Birmingham, AL) diluted in blocking buffer. Plates were developed with SIGMAFAST p-Nitrophenyl phosphate Tablets (Sigma-Aldrich), and specific absorbance was measured at 405 nm using a Molecular Devices SpectraMax micro-plate reader.
+ Open protocol
+ Expand
2

Quantifying Autoantibodies in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-MTg autoantibodies were determined by ELISA, as described in detail previously (36 (link)). Sera from individual mice were diluted 1/50 or 1/100 and plated in duplicate on MTg-coated ELISA plates. Anti-Ro and anti-La Abs were quantified by ELISA. Briefly, plates were coated overnight with 5 μg/ml anti-Ro Ab (Sigma-Aldrich, St. Louis, MO) or anti-La Ab (Abcam, Cambridge, MA) diluted in PBS. After washing with PBS with 0.5% Tween and blocking with 0.2% BSA in PBS, serum was diluted and incubated on plates overnight. Bound Ig was detected with goat anti-mouse IgG–alkaline phosphatase (0.2 μg/ml; Southern-Biotech, Birmingham, AL) diluted in blocking buffer. Plates were developed with SIGMAFAST p-Nitrophenyl phosphate Tablets (Sigma-Aldrich), and specific absorbance was measured at 405 nm using a Molecular Devices SpectraMax micro-plate reader.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!