Anti-MTg autoantibodies were determined by ELISA, as described in detail previously (36 (link)). Sera from individual mice were diluted 1/50 or 1/100 and plated in duplicate on MTg-coated ELISA plates. Anti-Ro and anti-La Abs were quantified by ELISA. Briefly, plates were coated overnight with 5 μg/ml anti-Ro Ab (Sigma-Aldrich, St. Louis, MO) or anti-La Ab (Abcam, Cambridge, MA) diluted in PBS. After washing with PBS with 0.5% Tween and blocking with 0.2% BSA in PBS, serum was diluted and incubated on plates overnight. Bound Ig was detected with goat anti-mouse IgG–alkaline phosphatase (0.2 μg/ml; Southern-Biotech, Birmingham, AL) diluted in blocking buffer. Plates were developed with SIGMAFAST p-Nitrophenyl phosphate Tablets (Sigma-Aldrich), and specific absorbance was measured at 405 nm using a Molecular Devices SpectraMax micro-plate reader.
Anti la ab
Manufactured by Abcam
Anti-La Ab is a laboratory product used for the detection and measurement of anti-La antibodies. Anti-La antibodies are autoantibodies that target the La/SSB protein, which is involved in RNA processing. This product can be used in various immunoassay techniques to aid in the diagnosis and monitoring of autoimmune disorders, such as Sjögren's syndrome and systemic lupus erythematosus.
Automatically generated - may contain errors
2 protocols using anti la ab
1
Quantifying Autoantibodies in Mice
2
Quantifying Autoantibodies in Mice
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Anti-MTg autoantibodies were determined by ELISA, as described in detail previously (36 (link)). Sera from individual mice were diluted 1/50 or 1/100 and plated in duplicate on MTg-coated ELISA plates. Anti-Ro and anti-La Abs were quantified by ELISA. Briefly, plates were coated overnight with 5 μg/ml anti-Ro Ab (Sigma-Aldrich, St. Louis, MO) or anti-La Ab (Abcam, Cambridge, MA) diluted in PBS. After washing with PBS with 0.5% Tween and blocking with 0.2% BSA in PBS, serum was diluted and incubated on plates overnight. Bound Ig was detected with goat anti-mouse IgG–alkaline phosphatase (0.2 μg/ml; Southern-Biotech, Birmingham, AL) diluted in blocking buffer. Plates were developed with SIGMAFAST p-Nitrophenyl phosphate Tablets (Sigma-Aldrich), and specific absorbance was measured at 405 nm using a Molecular Devices SpectraMax micro-plate reader.
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