Xterra rp18 reverse phase column

Manufactured by Waters Corporation

Sourced in United States

The XTerra RP18 is a reverse phase column used in liquid chromatography. It features a silica-based stationary phase with C18 alkyl ligands, providing a hydrophobic surface for the separation of nonpolar and moderately polar compounds.

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Lab products found in correlation

Agilent 1200 series hplc system, by Agilent Technologies (1 mentions) Agilent 1200 system, by Agilent Technologies (1 mentions)

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XTerra RP18 (50 citations) XTerra RP18 column (42 citations) XTerra (17 citations) Xterra column (12 citations)

2 protocols using xterra rp18 reverse phase column

HPLC Analysis of Sulforaphane Levels

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Chromatographic analysis of sulforaphane content was performed using an Agilent 1200 Series HPLC System (Agilent Technologies, Inc., Santa Clara, CA, USA) consisting of a solvent gradient delivery pump and a photodiode array detector. Separation was achieved using an XTerra RP18 reverse phase column (25 cm×4.6 mm; 5 μm; Waters, Milford, MA, USA). Acetonitrile (A) and water (B) were used as mobile phases and the gradient elution was programmed as follows: 0 min, 20% A; 10 min, 50% A; 15 min, 20% A. The flow rate was 0.8 mL/min and the detection wavelength was 240 nm. The injection volume was 20 μL.

Hwang J.H, & Lim S.B. (2014). Antioxidant and Anti-inflammatory Activities of Broccoli Florets in LPS-stimulated RAW 264.7 Cells. Preventive Nutrition and Food Science, 19(2), 89-97.

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HPLC Quantification of Mefloquine

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The HPLC method for the quantification of mefloquine was performed as previously described [28 (link)]. Briefly, HPLC was carried out using an Agilent 1200 system (Santa Clara, CA). A concentration gradient mefloquine at 0.19, 0.38, 0.75, 1 and 1.5 mg/mL was prepared. An Xterra RP18 reverse-phase column (250×4.6 mm, 5 mm particle size, Waters, Milford, MA) was adopted and maintained at 30 °C. The mobile phase consisted of 0.1 M monobasic sodium phosphate buffer (pH 3.0): methanol (35: 65, v/v), the flow rate was 1 mL/min, and the injection volume was 10 μL. Mefloquine was detected at the wavelength of 280 nm.

Qian J., Wang M., Zhang M., Feng R., Zhang J., Ye C., Wang B, & Cui L. (2021). Development and application of immunoassays for rapid quality control of the antimalarial drug combination artesunate-mefloquine. Journal of pharmaceutical and biomedical analysis, 207, 114342.

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