Ccl 163tm

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), antibiotics (penicillin and streptomycin), and Neutral Red were purchased from Sigma-Aldrich, Schnelldorf, Germany. The disposable consumables were supplied by Orange Scientific, Braine-l’Alleud, Belgium. The BALB/c 3T3 clone A31 (ATCC^® CCL-163^TM)—mouse embryonic fibroblast, MCF-10A (ATCC^® CRL-10317™)—normal human epithelial, PC3 (ATCC^® CRL-1435™)—prostate carcinoma, and HT-29 (ATCC^® HTB-38™)—colorectal adenocarcinoma cell lines were obtained from American Type Cultures Collection (ATCC, Manassas, VA, USA).
The in vitro tests were performed to determine the cytotoxicity and antiproliferative activity of glyceride oil on cell lines. The findings were mathematically, statistically, and graphically processed, suitable for publishing in specialized scientific journals.

The mice embryonal fibroblasts BALB/c 3T3, A31 (ATCC^® CCL-163TM); the non-tumorigenic cell line from epithelium of breast MCF-10A (ATCC^® CRL-10317™); the luminal adenocarcinoma from breast gland MCF-7 (ATCC^® HTB-22™), type A (ER+, PR+, HER2-), and the triple negative carcinoma of breast gland MDA-MB-231 (ATCC^® HTB-26™), (ER-, PR-, HER2-) were used. The cultivation of the adhered cell cultures was done in DMEM medium (4.5 g/L glucose), 10% fetal calf serum, 100 U/mL penicillin, and 0.1 mg/mL streptomycin in plastic dishes with a working area of 25 cm² and 75 cm². The cells were kept in logarithmic phase of grown at 37 °C in 5% CO₂ atmosphere. The cells’ samples for in vitro tests were prepared from the cells in an exponential stage of grown-up. After trypsinization, the cells were brought to the required concentration so that the cell density in each of the 96-well plates was fixed to 1 × 10⁴ cells per well. The cultivation was performed for 24 h. Then, the cells were incubated with compounds.