Leu gly pro ala

Collagenase from Clostridium histolyticum (Sigma Aldrich, Saint Quentin Fallavier, France) was used. The collagenase activity was determined using N-[3-(2-furyl) acryloyl]-Leu-Gly Pro-Ala (FALGPA; Sigma Aldrich, Saint Quentin Fallavier, France) as a substrate following the protocol of Wittenauer et al. [34 (link)]. Absorbance decrease was followed at 335 nm during 20 min thank to a microplate reader (BioTek ELX800; BioTek Instruments, Colmar, France). The collagenase activity in presence of each extraction conditions was determined in triplicated and the anti-collagenase activity was expressed, for each extract, as an inhibition percentage relative to corresponding control (adding same volume of extraction solvent).

Collagenase clostridium histolyticum (Sigma Aldrich) was employed for this assay and its activity was determined with the aid of a spectrophotometer by making use of N-[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA; Sigma Aldrich) as a substrate in accordance to the protocol of Wittenauer et al. (2015) [78 (link)]. The absorbance decrease of FALGPA was followed at 335 nm for 20 min using a microplate reader (BioTek ELX800; BioTek Instruments, Colmar, France). Triplicated measurements were used and the anti-collagenase activity was revealed as a % of inhibition relative to corresponding control (adding same volume of extraction solvent) for every extract.

Collagenase clostridium histolyticum (Sigma Aldrich) was used for this experiment, and its activity was determined with the aid of a spectrophotometer using N-[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA; Sigma Aldrich) as the substrate in accordance with the protocol of Wittenauer et al. [51 (link)]. The decrease in the absorbance of the FALGPA was followed for 20 min at 335 nm using a microplate reader (BioTek ELX800; BioTek Instruments, Colmar, France). The measurements were conducted in triplicate, and the anti-collagenase activity was revealed as the percent inhibition relative to the control (extraction solvent) for every extract sample. 1,10-Phenantroline (100 µM) was used as the specific inhibitor of collagenase.