[3H]RA solution in DMSO was used for the preparation of NPs, using a 1:10 ratio of labelled to unlabelled RA (1 nCi/μg RA). The initial RA cargo in the NPs was quantified using 2/3 of the original NP suspension (1 mg/mL). To quantify the controlled release of the RA, a 10 μg/mL suspension of [3H]RA-NPs was prepared and irradiated with a blue laser (405 nm, 80 mW/cm2). For each timepoint (0s, 60s, 180s, 300s, 600s) the NP suspension was centrifuged at 14,000 g for 3 min, the supernatant collected and mixed with liquid scintillation fluid (1 mL; Packard Ultima Gold) and the scintillations counted in a TriCarb 2900 TR Scintillation analyser (PerkinElmer). The amount of RA was determined by the linear regression equation y = 1.3985x (R2 = 0.9947).
Tricarb 2900 tr scintillation analyser
Manufactured by PerkinElmer
Sourced in United Kingdom
The TriCarb 2900 TR Scintillation Analyser is a versatile laboratory instrument used for the detection and quantification of radioactive samples. It employs liquid scintillation counting technology to measure the radioactive decay of samples. The TriCarb 2900 TR provides precise and accurate measurements of alpha, beta, and gamma radiation.
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2 protocols using tricarb 2900 tr scintillation analyser
1
Quantifying Controlled Release of Radiolabeled Retinoic Acid from Nanoparticles
2
Quantification of Light-Responsive Nanoparticle Release
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A solution of [ 3 H]RA in DMSO was used for the preparation of LR-NP, using a 1:20 ratio of labeled to unlabeled RA (1nCi μg -1 RA). The initial RA cargo was quantified using 2/3 of the original LR-NP suspension (1mg ml -1 ). To quantify the controlled release of RA, a 10μg mL -1 suspension of [ 3 H]LR-NP was irradiated with blue light (405 nm). For each timepoint (0s, 30s, 60s, 600s) the NP suspension was centrifuged at 14000g for 3 minutes, the supernatant collected and mixed with liquid scintillation fluid (1 mL; Ultima Gold, Packard Instrument SA, Rungis, France) and the scintillations counted in a TriCarb 2900 TR Scintillation analyser (Perkin Elmer, Buckinghamshire, UK).
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