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Alexafluor 800 secondary antibody

Manufactured by Thermo Fisher Scientific
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The AlexaFluor 800 secondary antibody is a fluorescent labeling reagent designed for use in various immunodetection techniques. It is a highly sensitive and specific detection reagent that binds to primary antibodies and emits fluorescence in the near-infrared spectrum, enabling visualization and quantification of target analytes.

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Lab products found in correlation

Ec6625box, by Thermo Fisher Scientific (2 mentions) Pierce 660 assay, by Thermo Fisher Scientific (2 mentions)

2 protocols using alexafluor 800 secondary antibody

1

TREM2 Cytosolic Domain Protein Quantification

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Protein was quantified using a Pierce 660 assay (Invitrogen #1861426) and 40 μg loaded per well on a 10–20% Tricine gel (Invitrogen #EC6625BOX) followed by transfer to a 0.22 μm PVDF membrane. The membrane was blotted overnight with an antibody against sequence within the TREM2 cytosolic domain (Cell Signaling #91068) and probed with a goat anti-rabbit secondary AlexaFluor 800 secondary antibody (Invitrogen #A32735).
Shaw B.C., Snider H.C., Turner A.K., Zajac D.J., Simpson J.F, & Estus S. (2022). An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brain. Journal of Alzheimer's disease : JAD, 87(4), 1647-1657.
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2

TREM2 Cytosolic Domain Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was quantified using a Pierce 660 assay (Invitrogen #1861426) and 40 µg loaded per well on a 10-20% Tricine gel (Invitrogen #EC6625BOX) followed by transfer to a 0.22 µm PVDF membrane. The membrane was blotted overnight with an antibody against sequence within the TREM2 cytosolic domain (Cell Signaling #91068) and probed with a goat anti-rabbit secondary AlexaFluor 800 secondary antibody (Invitrogen #A32735).
Shaw B.C., Snider H.C., Turner A., Zajac D.J., Simpson J.F., & Estus S. (2021). An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brain.
+ Open protocol
+ Expand

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