Hydrogenated soybean l α phosphatidylcholine hspc 1 2 distearoyl sn glycero 3 phosphoethanolamine n methoxy polyethyleneglycol 2000 dspem peg2000

Cisplatin, mifepristone, trypsin, sodium chloride, nickel chloride, and sodium diethyl dithiocarbamate (DDTC) were supplied by Sigma–Aldrich Chemical Co. (St. Louis, MO, USA). Hydrogenated soybean L-α-phosphatidylcholine (HSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DSPEm-PEG2000), and cholesterol were purchased from Avanti Polar Lipids (Birmingham, AL, USA). High-performance liquid chromatography (HPLC)-grade acetonitrile, chloroform, and methanol were acquired from Honeywell International, Inc. (Morristown, NJ, USA). Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific Inc., Waltham, MA, USA), fetal calf serum (FCS, Thermo Fisher Scientific Inc., Waltham, MA, USA), ethylenediaminetetraacetic acid (EDTA, Thermo Fisher Scientific Inc., Waltham, MA, USA), Tris, and SDS were procured from GIBCO Inc. (Grand Island Biological Company, New York, NY, USA). High-quality water employed to prepare solutions was obtained with a Continental Milli-Q Reagent Water System (Millipore, El Paso, TX, USA).

Cisplatin, ferrous ammonium sulphate, ferrozine reagent, nickel chloride, potassium chloride, sodium acetate, sodium chloride, and sodium diethyldithiocarbamate (DDTC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Uranyl acetate was acquired from SPI Supplies (West Chester, PA, USA). Hydrogenated soybean l-α-phosphatidylcholine (HSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DSPE-mPEG₂₀₀₀) and cholesterol were obtained from Avanti Polar Lipids (Birmingham, AL, USA). Oleic-acid-coated iron oxide (Fe₃O₄) nanoparticles (magnetite nanoparticles) with a mean hydrodynamic diameter of 10 nm and magnetization superior to 45 emu/g were also purchased from Sigma-Aldrich.