Female cd 1 mice

Epyrifenacil and S-3100-CA were synthesized in our laboratory. The chemical purity of these compounds was determined to be 96.8% and 99.7% (HPLC), respectively. Phenyl-14 C-labeled S-3100-CA was also synthesized in our laboratory and had a specific activity of 4.26 GBq/mmol with radiochemical purity of 97.8% (radio-HPLC). The structures of these compounds are shown in Fig. 1. α-Cyano-4-hydroxycinnamic acid (α-CHCA) was supplied by Merck KGaA (Darmstadt, Germany). PPIX analytical standard was supplied by Frontier Scientific (Logan, UT, USA). Rifampicin was supplied by FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Digoxin was supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Krebs-Henseleit buffer (KHB), silicone oil, and mineral oil were purchased from Merck KGaA. The hepatocyte isolation kit was supplied by Sekisui XenoTech, LLC (Kansas City, KS, USA). Cryopreserved primary hepatocytes of male SD rats (pool of 8), female SD rats (pool of 12), male CD-1 mice (pool of 16), and female CD-1 mice (pool of 23) were from Sekisui XenoTech, LLC. Cryopreserved primary hepatocytes of humans (pool of 10 males and 10 females) were purchased from Biopredic International (Saint Grégoire, France). Other chemicals were of reagent grade.