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3 protocols using mouse monoclonal anti hemagglutinin ha antibody

1

Antibody Sources for Western Blotting

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Mouse monoclonal anti-hemagglutinin (HA) antibody was purchased from Covance Inc. (Berkeley, CA); rabbit polyclonal anti-HA, monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and monoclonal anti-GFP antibodies were obtained from Abcam Inc. (Cambridge, MA); mouse monoclonal anti-Flag M2 antibody was from Sigma; rabbit polyclonal anti-GFP antibody was from Life Technologies. Mouse monoclonal anti-human transferrin antibody was from Invitrogen. Mouse monoclonal anti-β-tubulin was from Sigma. Mouse monoclonal anti-ubiquitin antibody (P4D1) was from Santa Cruz Biotechnology. Rabbit polyclonal anti-cleaved caspase-3 (Asp175) antibody was purchased from Cell Signaling Technology (kindly provided by Dr. Peter Siegel, McGill University). Horseradish peroxidase-conjugated secondary IgG antibodies, as well as FITC-conjugated goat anti-mouse secondary Fab were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA). All Alexa Fluor® conjugated secondary antibodies were purchased from Molecular Probes (Eugene, OR). Alpha-minimum essential medium (α-MEM), fetal bovine serum, penicillin/streptomycin, and trypsin-EDTA were purchased from Wisent (Saint-Bruno, QC, Canada). The DMEM/F12 medium was from Corning. All other chemical and reagents were obtained from BioShop Canada (Burlington, ON, Canada), Sigma or Fisher Scientific and were of the highest grade available.
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2

CFTR Antibodies and Correctors Protocol

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Mouse monoclonal anti–hemagglutinin (HA) antibody was purchased from Covance. Monoclonal anti-CFTR antibody M3A7 (recognizing aa 1365–1395 at the C terminus of the NBD2) and MM13–4 mouse monoclonal anti-CFTR Ab (specific to the N-terminal 25–36 residues) were from Millipore. The 660 anti-CFTR antibody, recognizing the NBD114, was kindly provided by Dr. J. Riordan (University of North Carolina, Chapel Hill, NC). VX-770 and VX-809 were purchased from Selleckchem. For follow up studies, the small molecule corrector compounds were acquired from Life chemicals (4172 and 3151 series as well as BIA29) or Maybridge (6258 series) and their identity was confirmed by mass spectrometry. All other chemicals were purchased from Sigma-Aldrich at the highest grade available.
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3

CFTR Antibodies and Correctors Protocol

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Mouse monoclonal anti–hemagglutinin (HA) antibody was purchased from Covance. Monoclonal anti-CFTR antibody M3A7 (recognizing aa 1365–1395 at the C terminus of the NBD2) and MM13–4 mouse monoclonal anti-CFTR Ab (specific to the N-terminal 25–36 residues) were from Millipore. The 660 anti-CFTR antibody, recognizing the NBD114, was kindly provided by Dr. J. Riordan (University of North Carolina, Chapel Hill, NC). VX-770 and VX-809 were purchased from Selleckchem. For follow up studies, the small molecule corrector compounds were acquired from Life chemicals (4172 and 3151 series as well as BIA29) or Maybridge (6258 series) and their identity was confirmed by mass spectrometry. All other chemicals were purchased from Sigma-Aldrich at the highest grade available.
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