1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine

Manufactured by Avanti Polar Lipids

Sourced in United States

1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine is a phospholipid consisting of a glycerol backbone with a palmitic acid and an oleic acid esterified to the first and second carbons, respectively, and a phosphocholine group attached to the third carbon. This compound is a commonly used lipid in various biochemical and biophysical applications.

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Lab products found in correlation

224 protocols using 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine

Giant Unilamellar Vesicle Formation

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The lipids 1-palmitoyl-2oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPS), cholesterol (chol), 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′-phosphate) (PI(3)P), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) and 1,2-dioleoyl-sn-glycero-3-phopho-(1′rac′glycerol) (DOPG) were purchased from Avanti Polar Lipids. In all cases, we added 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (TexasRed-DHPE) (Molecular Probes) at a concentration of 0.1 mol% in the lipid mixtures for the visualization of the membranes. Giant unilamellar vesicles of different lipid composition were grown using the electroformation method (Angelova and Dimitrov, 1986 (link)). Briefly, 10 μl of a 4 mM lipid stock solution in chloroform were spread on indium tin oxide (ITO) coated glasses. The excess of chloroform was eliminated under vacuum at room temperature (RT) for 1h. Then the glasses were assembled with a 2 mm-thick Teflon spacer between them to form the electroformation chamber, which was filled with a 600 mM sucrose solution that matched the osmolarity of the buffer containing the proteins (~650 mOsmol). Finally, an electric AC-field (1.6V, 10 Hz) was applied for 1 h at different temperatures. GUVs were collected and cooled to RT before use.

Avalos-Padilla Y., Knorr R.L., Javier-Reyna R., García-Rivera G., Lipowsky R., Dimova R, & Orozco E. (2018). The Conserved ESCRT-III Machinery Participates in the Phagocytosis of Entamoeba histolytica. Frontiers in Cellular and Infection Microbiology, 8, 53.

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HUVEC Culture and VEGF Peptide Screening

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Human umbilical vein endothelial cells (HUVECs) and cell culture media (EGM^®-2 BulletKit^®, culture system containing EBM-2 (Endothelial Cell Growth Basal Medium) and EGM-2 SingleQuots supplements required for growth of endothelial cells) were purchased from Lonza srl (Milan, Italy). Recombinant Human VEGF165 was obtained from PeproTech (5 Crescent Ave, Rocky Hill, NJ, USA). Bathocuproine disulfonic acid disodium salt and 6-carboxyfluorescein were purchased from Fluka, Sigma-Aldrich (Milan (MI), Italy). VEGF73-101 and VEGF84-101 were synthesized as reported in previous work [32 (link)], while VEGFQ79G and VEGFI83G were purchased from Genecust (Dudelange, Luxembourg). N- and C-terminally blocked fragments. 1,2-palmitoyl-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-palmitoyl-oleoyl-sn-glycero-3-phosphocholine (POPE) and 1,2-palmitoyl-oleoyl-sn-glycero-3-phospho-L-serine (POPS) were purchased from Avanti Polar Lipids (Alabaster Alabama (AL), USA) with a purity of 99% and used without further purification.

Zimbone S., Santoro A.M., La Mendola D., Giacomelli C., Trincavelli M.L., Tomasello M.F., Milardi D., García-Viñuales S., Sciacca M.F., Martini C, & Grasso G. (2020). The Ionophoric Activity of a Pro-Apoptotic VEGF165 Fragment on HUVEC Cells. International Journal of Molecular Sciences, 21(8), 2866.

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Lipid Preparation for Neutron Scattering

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D₂O (99.9% deuterated, Sigma Aldrich) was provided by ISIS Neutron and Muon Source. Ultrapure Milli-Q (MQ) water was used for all cleaning procedures and solvents. Tris buffer was prepared by dissolving a tablet of 50 mM Tris, 150 mM NaCl, pH 7.4 as specified by the producer (Sigma-Aldrich) in either D₂O or H₂O. Lipid standards and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were purchased from Avanti Polar Lipids (Alabaster, AL).

Maric S., Lind T.K., Raida M.R., Bengtsson E., Fredrikson G.N., Rogers S., Moulin M., Haertlein M., Forsyth V.T., Wenk M.R., Pomorski T.G., Arnebrant T., Lund R, & Cárdenas M. (2019). Time-resolved small-angle neutron scattering as a probe for the dynamics of lipid exchange between human lipoproteins and naturally derived membranes. Scientific Reports, 9, 7591.

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Purification and Production of KRAS4b

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Fully processed KRAS4b was produced and purified as described before (85 (link)). All lipids were purchased from Avanti Polar Lipids (Alabaster): 1,2-dioleoyl-sn-glycero-3 phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine sodium salt (DOPS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine sodium salt (POPS).

Liang H., Mu H., Jean-Francois F., Lakshman B., Sarkar-Banerjee S., Zhuang Y., Zeng Y., Gao W., Zaske A.M., Nissley D.V., Gorfe A.A., Zhao W, & Zhou Y. (2019). Membrane curvature sensing of the lipid-anchored K-Ras small GTPase. Life Science Alliance, 2(4), e201900343.

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Synthetic Pln149 Peptide Purification

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Materials -Synthetic Pln149 was produced by solid phase peptide synthesis using Fmoc chemistry, and then purified by reverse phase chromatography, as described in Lopes et al. (2009) (link). The purity (>95%) and molecular weight (2423.7
Da) of Pln149 were determined by mass spectrometry (Lopes et al., 2009) (link). Phospholipids 1-palmitoyl-2-oleoyl-snglycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), and 1-palmitoyl-2oleoyl-sn-glycero-3-phospho-L-serine (POPS) were purchased from Avanti Polar Lipids and reagents were P.A. grade.

Kumagai P.S., Sousa V.K., Donato M., Itri R., Beltramini L.M., Araujo A.P.U., Buerck J., Wallace B.A, & Lopes J.L.S. (2019). Unveiling the binding and orientation of the antimicrobial peptide Plantaricin 149 in zwitterionic and negatively charged membranes. European biophysics journal : EBJ, 48(7).

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Lipid-based Nanoparticle Formulation Protocol

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Soybean oil and Myrj™ s40 (PEGylated surfactant) were purchased from Croda, Chocques, France. Lipoid S75 and dipalmitoylphosphatidylcholine were from Lipoid GmbH, Ludwigshafen, Germany. Suppocire NB was from Gatefosse, Saint-Priest, France. 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), cholesterol, 1,2-distearoyl-sn-glycero-3-phospho-ethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-PEG-2000) and 1,2-distearoyl-phosphatidylethanolamine-methyl-polyethyleneglycol conjugate-550 (DSPE-PEG550) were purchased from Avanti Polar Lipids, Inc. (AL, USA).

Matuszak J., Baumgartner J., Zaloga J., Juenet M., da Silva A.E., Franke D., Almer G., Texier I., Faivre D., Metselaar J.M., Navarro F.P., Chauvierre C., Prassl R., Dézsi L., Urbanics R., Alexiou C., Mangge H., Szebeni J., Letourneur D, & Cicha I. (2016). Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing. Nanomedicine (London, England), 11(6).

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Isolation of Translocated TccC3HVR

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To isolate translocated TccC3HVR, we assembled ABC wild-type holotoxin (700 nM) as described above, and triggered the prepore-to-pore transition by dialysis against 20 mM CAPS-NaOH pH 11.2, 150 mM NaCl in the presence of 9 μM Msp1D1 nanodisc scaffold protein (Cube Biotech, Cat. No. 26112) and 490 μM 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids, Cat. No. 850457 C) dissolved in 1% sodium cholate. After 72 h of dialysis at 4 °C, we subjected the holotoxin to SEC on a Superose 6 increase 5/150 column equilibrated in dialysis buffer and collected the fractions after the holotoxin peak (see Supplementary Fig. 3a).

Roderer D., Schubert E., Sitsel O, & Raunser S. (2019). Towards the application of Tc toxins as a universal protein translocation system. Nature Communications, 10, 5263.

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Lipid Bilayer Formation and Characterization

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The lipids used in the study were 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), purchased from Avanti Polar Lipids; porcine globotriaosylceramide (Gb 3 ) was purchased from Matreya LLC. The Shiga toxin B unit (STxB) was supplied by Prof. Ludger Johannes, Institut Curie, UMR144 Curie/ CNRS, Paris, France. 8 All lipids were dissolved in methanol before sample preparation. All used chemicals were of HPLC grade quality and Milli-Q water was used for the buffer preparation. HEPES buffer (5 mM, 150 mM NaCl), pH7.0 was prepared by mixing HEPES acid, HEPES base and NaCl components. Muscovite mica from Plano GmbH (Germany) was cut into approx. 10 Â 10 mm 2 pieces, freshly cleaved, and used as a substrate.

Solovyeva V., Johannes L, & Simonsen A.C. (2015). Shiga toxin induces membrane reorganization and formation of long range lipid order. Soft matter, 11(1).

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Lipid Composition Analysis Protocol

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Materials 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-d31-2-oleoyl-sn-glycero-3-phosphocholine (dPOPC), 1,2-dipalmitoyl-d31sn-glycero-3-phosphocholine (dDPPC), 1-palmitoyl-d31-2-oleoyl-snglycero-3-phosphoethanolamine (dPOPE) and sphingomyelin (SM) were from Avanti Polar Lipids. Cholesterol-3,4-13 C2 ( 13 C-chol) was from Cambridge Isotope Laboratory. All other chemicals were from Sigma, unless stated otherwise.

Kozorog M., Sani M.A., Separovic F, & Anderluh G. (2018). Listeriolysin O Binding Affects Cholesterol and Phospholipid Acyl Chain Dynamics in Fluid Cholesterol-Rich Bilayers. Chemistry (Weinheim an der Bergstrasse, Germany), 24(53).

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Lipid Membrane Characterization using Diverse Particles

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1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), porcine brain sphingomyelin (BSM), ovine wool cholesterol (Chol), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-lissamine rhodamine B sulfonyl 18:1 (Liss Rhod PE), N-[11-(dipyrrometheneboron difluoride)undecanoyl]-D-erythro-sphingosylphosphorylcholine (C11 TopFluor SM), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DOPE-PEG 2000) were purchased from Avanti Polar Lipids.
Silica spheres were purchased from Microparticles GmbH (2.1 ± 0.1 μm and 7.0 ± 0.3 μm). Polystyrene-3-(Trimethoxysilyl)propyl methacrylate (PS-TPM) dumbbell and snowman particles were synthesised by making a protrusion from swollen polystyrene particles^{42 (link)}. Hematite cubic particles were made following the method of⁴³ and coated with silica⁴⁴. Their corner-to-corner distance was 1.8 ± 0.1 μm. All errors reported for particle sizes are standard deviations.
HEPES buffer was made with 115 mM NaCl, 1.2 mM CaCl₂, 1.2 mM MgCl₂, 2.4 mM K₂HPO₄, and 20 mM HEPES.

Rinaldin M., Fonda P., Giomi L, & Kraft D.J. (2020). Geometric pinning and antimixing in scaffolded lipid vesicles. Nature Communications, 11, 4314.

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