P stat3 luc

A STAT3 luciferase construct (p-STAT3-luc) driven by a quadrupled STAT3 response element was obtained from Beyotime Biotechnology Institute (Nantong, China). A cyclin D2 luciferase reporter plasmid was established in pGL4 vector (Promega) driven by a fragment of cyclin D2 promoter containing or lacking a STAT3 response element [21 (link)]. A NF-κB luciferase reporter construct (pNF-κB-Luc) was provided by Clontech Laboratories.

The human transcription factor STAT3 cDNA was cloned into pcDNA3.1 vector with a Myc tag generously as described previously [8 (link)]. The regulatory sequences of cyclin D2 promoter with or without a STAT3 response element was inserted into pGL4 vector (Promega Corporation, Madison, WI, USA) as previously described [34 (link)]. A STAT3-driving luciferase construct (pSTAT3-luc) and a NF-κB luciferase construct (pNF-κB-Luc) were purchased from Beyotime Biotechnology Institute (Nantong, China) [8 (link)]. Plasmids were transiently transfected into HeLa or RPMI-8226 cells by Lipofectamine^® 2000 (Invitrogen) according to the manufacturer's instruction.