Corneas and submandibular lymph nodes (MLN) were homogenized with a Tissuemiser (Fisher Scientific, Pittsburgh, PA, USA) in the presence of 1× Calbiochem protease inhibitor cocktail set I (EMD Millipore, Billerica, MA, USA ) in 1× PBS. The tissue homogenates were centrifuged at 10,000 × g for 3 minutes at 4°C. Supernatants were collected and assessed for VEGF-A content using a standard ELISA kit (R&D Systems, Minneapolis, MN, USA) or IL-2, IL-7, IL-12 p70, and IL-15 content using a multiplex suspension array kit (EMD Millipore). VEGF-A ELISA plates were analyzed using a CLARIO star microplate reader (BMG LABTECH, Cary, NC, USA). The luminex x-map bead-based multiplex assays were analyzed using a Bio-plex 200 system (Bio-Rad, Hercules, CA, USA).
Multiplex suspension array kit
Manufactured by Merck Group
Sourced in United States
The Multiplex suspension array kit is a laboratory equipment product designed for high-throughput analysis of multiple analytes in a single sample. It utilizes color-coded beads to enable simultaneous detection and quantification of various biomolecules, such as proteins, cytokines, or nucleic acids. The core function of this kit is to provide a versatile and efficient platform for multiplexed assays, allowing researchers to gather comprehensive data from limited sample volumes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using multiplex suspension array kit
1
Quantification of Immune Factors in Corneal and Lymph Node Tissues
2
Quantification of Immune Factors in Corneal and Lymph Node Tissues
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