MTT was purchased from Sigma‐Aldrich (USA). Chloroquine diphosphate, E‐64, PKI‐402 and rapamycin were purchased from MedChemExpress (USA). The primary antibodies against LC3B, SQSTM1/p62, TFEB, PINK1, parkin, Fis1, Drp1, Mfn1 and Mfn2 were purchased from Abcam (USA), and LAMP1, beta‐actin, alpha‐tubulin, cathepsin B, cathepsin D and cytochrome c were purchased from Proteintech (USA).
Pki 402
Manufactured by MedChemExpress
Sourced in United States
PKI-402 is a laboratory compound used for research purposes. It functions as a potent and selective inhibitor of Protein Kinase Inhibitor-402, a kinase enzyme involved in cellular signaling pathways. This product is intended for use in scientific research and evaluation, not for use in humans or animals.
Automatically generated - may contain errors
Lab products found in correlation
Sqstm1 p62, by Abcam (1 mentions)
Parkin, by Abcam (1 mentions)
β actin, by Proteintech (1 mentions)
Cathepsin d, by Proteintech (1 mentions)
Cytochrome c, by Proteintech (1 mentions)
Pink1, by Abcam (1 mentions)
Chloroquine diphosphate, by MedChemExpress (1 mentions)
Primary antibodies against lc3b, by Abcam (1 mentions)
Lamp1, by Proteintech (1 mentions)
Cathepsin b, by Proteintech (1 mentions)
Alpha tubulin, by Proteintech (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Rapamycin, by MedChemExpress (1 mentions)
Infigratinib, by MedChemExpress (1 mentions)
2 protocols using pki 402
1
Autophagy and Mitochondrial Dynamics Assay
2
Cytotoxicity Evaluation of Infigratinib and PKI-402
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Infigratinib (HY-13311) and PKI-402 (HY-10683, MedChemexpress, NJ, USA) were prepared to make stock solutions in DMSO. Both agents were then dissolved in 1X phosphate buffer saline (1X PBS) in cell culture treatment conditions. CCA cells were seeded at a 5000 cells/well density in 96-well plates overnight. Then, the cells were treated with various concentrations of each agent for 72 h. Cell viability was assessed using the SRB (3520-42-1, Sigma-Aldrich Pte Ltd, MO, USA) assay, and the absorbance was measured at 540 nm. Control cells were cultured in full media with 0.05 % DMSO for the entire culture process. The IC50 of cytotoxicity was evaluated using GraphPad Prism version 8.0 (La Jolla, CA, USA).
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