All reagents were purchased from Sigma-Aldrich, and used without further purification: silver nitrate (nr. 209139, ACS reagent, ≥99.0%), tannic acid (nr. 403040, ACS reagent), sodium citrate tribasic dehydrate (nr. 71402, BioUltra, ≥99.5%), polyvinylpyrrolidone (nr. PVP10, average m.w. 10 kDa). All glassware employed for AgNP preparation was cleaned with conc. HNO3 and rinsed with plenty of water. Ultrapure deionized water (Millipore purification system, 18.2 MΩ cm) was used for the preparation of all aqueous solutions. All solutions used for nanoparticle preparation were filtered through a 0.2 μm membrane filter (Whatman, cellulose acetate). AgNPs were characterized by Dynamic Light Scattering (DLS) (Zetasizer Nano ZS, Malvern Instruments). AgNP diameter was measured via TEM (JEOL JEM-1011 transmission electron microscope operating at an accelerating voltage of 100 kV). UV/Vis spectra and fluorescence measurements were carried out using a TECAN Infinite M200 Pro plate reader. The concentration of silver in the inks was determined via ICP-OES (Agilent 720 ICP-OES).
Cellulose acetate
Manufactured by Cytiva
Cellulose acetate is a lab equipment product that serves as a material for various applications. It is a synthetic polymer derived from cellulose, a naturally occurring organic compound. Cellulose acetate has properties such as transparency, durability, and chemical resistance, making it suitable for use in various laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Icp oes 720, by Agilent Technologies (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Infinite m200 pro, by Tecan (1 mentions)
Jem 1011, by JEOL (1 mentions)
Agilent 5975c msd, by Agilent Technologies (1 mentions)
Agilent 6890, by Agilent Technologies (1 mentions)
Cellulose nitrate, by Cytiva (1 mentions)
Zymolyase 20t, by MP Biomedicals (1 mentions)
6 protocols using cellulose acetate
1
Synthesis and Characterization of Silver Nanoparticles
2
Measuring Isotopic DIC Fractions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isotopic fractions of DIC in the liquid media were measured based on a modified headspace method [49 ]. 3 ml of culture liquid were collected from the bioreactor with a syringe and directly filtered through a sterile 0.45 µm filter (Whatman, cellulose acetate) and 26 G needle into a 60 ml bottle containing 1 ml 6 M HCl and crimp sealed with a rubber stopper. Prior to adding the liquid sample, bottles and HCl were flushed with either 100% N2 or Ar gas to void the headspace of background CO2. Samples were equilibrated with the acid in the bottles for at least 1 hour at room temperature to drive all DIC into the gas phase. 50 µl of the bottles headspace was then injected with a gas tight syringe (Hamilton) into a gas chromatograph (Agilent 6890 equipped with 6 ft Porapak Q columns) at 80 °C with helium as a carrier gas at a flow rate of 24 ml min−1, coupled to a mass spectrometer (Agilent 5975 C MSD; Agilent, Santa Clara, CA) to determine the isotopic fractions of 12CO2 and 13CO2.
Reactor headspace gas samples were collected manually using a gas tight syringe and needle (Hamilton) through a rubber septum in the reactor headplate and directly injected into the GC-MS as described above.
Reactor headspace gas samples were collected manually using a gas tight syringe and needle (Hamilton) through a rubber septum in the reactor headplate and directly injected into the GC-MS as described above.
3
Filtration System Comparison for Microparticle Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A complete filtration system of 25 mm (Product no. 073-0Q7724, Scharlab) was used for filtration. The filters assessed were polycarbonate cyclopore track etched (Catalogue no. 7060-2504, Whatman), nylon (Catalogue no. 7404-002, Whatman), cellulose acetate (Catalogue no. 10404006, Whatman), cellulose nitrate (Catalogue no. 10401106, Whatman), and mixed cellulose ester (Ref. HAWP02500, Merck Millipore Ltd), in all instances 0.45-μm pore size and 25-mm diameter. The magnetic actuation was achieved with the 16-tube magnet (Product no. 12321D, Thermo Fisher Scientific). The electrochemical readout was achieved on carbon screen-printed electrodes (ref. DRP-C110) using a portable bipotentiostat DRP-STAT200 operated by DropView 200 for instrument control and data acquisition (Dropsens, Spain). The scanning electron microscope (SEM) images were taken with the EVO MA-10 (with EDS Detector, Oxford LINCA).
4
Quantitative Vitamin C Analysis in Peppers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Vitamin C content was determined according to methods and HPLC protocols of Nagy et al. (2015) (link). About 5 grams of homogenised pepper fruit (seed excluded) was crushed in a crucible mortar with quartz sand. 50 mL of 3% metaphosphoric acid solution was gradually added to the mixture. The mixture was filtered through a filter paper and further purified by passing it through a 0.45 mm cellulose acetate (Whatman) syringe filter before it was injected into an HPLC. For the quantitative determination of ascorbic acid, sample data were compared to that generated using standard materials (Sigma-Aldrich, Budapest, Hungary).
5
Virus-Like Particle Harvest and Cell Lysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
2 days after transfection, VLPs were harvested from the medium of one well of a 6-well plate. The medium was filtrated (0.45-μm pore size, cellulose acetate, Whatman/GE Healthcare) to remove debris. VLPs were collected by high-speed centrifugation (232,000 × g, 30 min), and the pellet was resuspended in sample buffer. For cell lysate preparation, cells were disrupted by incubation with 50 mm Tris (pH 7.5), 1% Triton, 0.1% SDS, 5 mm EDTA, and protease inhibitors for 15 min on ice. Debris was removed by centrifugation at 16,000 × g for 10 min. The supernatant was subjected to TCA precipitation.
6
Yeast Spheroplast Isolation and Virus-Like Particle Harvest
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After harvesting, yeast cells were washed in water. Spheroplasts were prepared from 5 OD600 cells by incubating the cells with 10 mm DTT and 50 mm Hepes (pH 7.5) for 10 min at 30 °C, removing DTT by washing the cells two times with 1 m sorbitol, and incubating the cells with Zymolyase 20T (0.3 mg/ml, MP Biomedicals) in SD medium buffered with 50 mm Hepes (pH 7.5) containing 1 m sorbitol for 30 min. To follow the progress of cell wall disruption, aliquots were resuspended in water, and burst of spheroplasts was detected by measuring the OD600. Zymolyase was afterward removed by washing the spheroplasts two times in SD medium containing 1 m sorbitol. Spheroplasts were incubated in SD medium containing 1 m sorbitol for the indicated times, and VLPs were harvested from the medium. Medium was taken after careful spheroplast sedimentation by using the short spin button of the table centrifuge and filtrated (0.45-μm pore size, cellulose acetate, Whatman/GE Healthcare) to remove debris. VLPs were collected by high-speed centrifugation (232,000 × g, 30 min), and the pellet was resuspended in sample buffer. At the last time point, the spheroplasts were lysed in sample buffer.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!