Particle morphological analysis was conducted by using transmission electron microscopy (TEM) (Philips CM-10, Eindhoven, The Netherlands). Formvar-coated copper grid (Plano GmbH, Wetzlar, Germany) was dipped into the NPs suspension, removed and air dried followed by staining with 2% w/v phosphor-tungstic acid solution. Digital Micrograph and Soft Imaging Viewer software (Olympus, Singapore) were used for image capturing and analysis.
Soft imaging viewer software
Manufactured by Olympus
Sourced in Singapore
The Soft Imaging Viewer software is a tool for viewing and analyzing digital microscope images. It provides basic functionality for image viewing, measurement, and annotation.
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Lab products found in correlation
3 protocols using soft imaging viewer software
1
Particle Morphology Analysis by TEM
2
Nanoparticle Morphological Analysis by TEM
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Morphological analysis was performed by transmission electron microscopy (TEM; TOPCON002B, Tokyo, Japan, accelerating voltage 200 kV). Formvar-coated copper grid (Plano GmbH, Wetzlar, Germany) was soaked with NPs suspension and dried in open air and then stained with phosphotungstic acid solution (2% w/v). Image capture and analysis were performed by Digital Micrograph and Soft Imaging Viewer software (Olympus, Singapore).
3
Immunofluorescence Microscopy of Transfected Cells
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Cells were processed for immunofluorescence 24–48 h after transfection. Cells grown on collagen-coated glass coverslips were fixed for 20 min with 4% (w/v) para-formaldehyde in PBS (pH 7.4), permeabilized with 0.2% (w/v) Triton X-100 for 10 min and blocked with 1% BSA for 10 min. Blocked cells were sequentially incubated with primary and secondary antibodies for 1 h in a humid chamber. Coverslips were washed with ddH2O to remove PBS and mounted with Mowiol medium (containing 25% n-propyl-gallate as anti-fading reagent) on glass slides. All immunofluorescence steps were performed at room temperature and cells were washed three times with PBS, pH 7.4 between each individual step. Cell imaging was performed using an Olympus IX81 microscope equipped with an UPlanSApo 100×/1.40 oil objective (Olympus). Digital images were taken with a CoolSNAP HQ2 CCD camera. Representative images only were adjusted for contrast and brightness using the Olympus Soft Imaging Viewer software (Olympus) and MetaMorph 7 (Molecular Devices).
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