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Zorbax 250 column

Manufactured by Agilent Technologies
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The Zorbax 250 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of chemical compounds. The column features a silica-based stationary phase and is available in various pore sizes and particle sizes to meet the specific needs of different analytical applications.

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Lab products found in correlation

Haase, by Merck Group (1 mentions)

Close spelling variants of this product

Zorbax Bio Series GF-250 column (4 citations) Zorbax GF-250 column (4 citations) Zorbax SB-C18 4.6 × 250 mm column (2 citations)

2 protocols using zorbax 250 column

1

Determining RpaL Biomolecular Structure

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Size exclusion chromatography (SEC) was used to determine the biological configuration of purified RpaL. An HPLC 1660 was used with an Agilent Zorbax 250 column. Phosphate-buffered solution (PBS) degassed with helium was used as the mobile phase and run at a flow rate of 0.4 ml/min and a run time of 10 min. Purified RpaL was buffer exchanged after purification into PBS. An injection volume of 25 μl was used.
Hickman T.W., Baud D., Benhamou L., Hailes H.C, & Ward J.M. (2020). Characterisation of four hotdog-fold thioesterases for their implementation in a novel organic acid production system. Applied Microbiology and Biotechnology, 104(10), 4397-4406.
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2

Hyaluronidase-Mediated Hyaluronan Degradation

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HA-OVA or HA diluted in DPBS (1 mg/mL) was incubated with hyaluronidase (HAase; Sigma-Aldrich, 10 mg/mL) at a ratio of 25:1 (w/w) for up to 24 h at 37 °C. Samples were analyzed by SEC-HPLC at different time points, employing an Agilent HPLC quaternary pump (Agilent) with an ultraviolet detector set at 210 nm. An isocratic method was used with 0.1 M PBS solution (pH 7, 1 mL/min flow rate) as the mobile phase and a Zorbax-250 column (4 μm, 9.4 × 250 mm; Agilent) as the stationary phase.
Dalla Pietà A., Carpanese D., Grigoletto A., Tosi A., Dalla Santa S., Pedersen G.K., Christensen D., Meléndez-Alafort L., Barbieri V., De Benedictis P., Pasut G., Montagner I.M, & Rosato A. (2021). Hyaluronan is a natural and effective immunological adjuvant for protein-based vaccines. Cellular and Molecular Immunology, 18(5), 1197-1210.
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