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2 protocols using anti pr set7

1

Comprehensive Antibody Resource for Liver Research

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The antibodies used in this study were from the following: Santa Cruz Biotechnology: anti-HNF4 (sc-8987), anti-AFP (sc-8108), anti-Pr-Set7 (sc-135009), anti-53BP1 (sc-22760) and anti-FGF7 (SC-27127); Cell Signaling Technology: anti-cyclin B1 (#4138), anti-Stat3 (#9132), anti-phospho-Stat3 (#9145) and anti-phospho-histone H2A.X (#9718); Abcam: anti-Ki67 (ab15580), anti-histone H4 mono methyl K20 (ab9051), anti-histone H4 tri methyl K20 (ab9053) and anti-Pr-Set7 (ab3798); Bethyl Laboratories, anti-Alb (A90–234); AbD Serotec, anti-F4/80 (MCA497); Merck-Millipore, anti-Sox9 (AB5535) and anti-Prominin-CD133 (MAB4310); Biolegend, anti-CD45 (#103101); and DAKO, anti-CK19 (#A0575). The A6 antibody was obtained from Valentina Factor (NIH).
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2

Immunohistochemical Analysis of Infection

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Animals were killed at the post-infection times indicated and subjected to full necropsy for histological and immunohistochemical analyses. Sections were blocked with 4% normal goat serum in PBS with 0.2% Triton for 2 h at RT before incubating overnight at 4 ℃ with the properly diluted antibodies: anti-PRSet7 1:100 (2996, CellSignaling); anti-γ tubulin 1:5, 000 (ab11317, Abcam); anti-pericentrin 1:2, 000 (ab4448, Abcam).
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