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Anti tfrc

Manufactured by ABclonal
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Sourced in United States

Anti-TFRC is a laboratory equipment product manufactured by ABclonal. It functions as an antibody that specifically recognizes and binds to the transferrin receptor (TFRC) protein.

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Lab products found in correlation

Anti slc7a11, by ABclonal (2 mentions) Anti gpx4, by Proteintech (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Ferrostatin 1, by Topscience (1 mentions) Anti gpx4, by ABclonal (1 mentions) Anti vcam 1, by ABclonal (1 mentions) Anti il 1β, by ABclonal (1 mentions) Anti icam 1, by ABclonal (1 mentions) Anti parkin, by ABclonal (1 mentions) Erastin, by Topscience (1 mentions) Anti tom20, by ABclonal (1 mentions) Anti cdk4, by Abcam (1 mentions) Odyssey infrared scanning system, by LI COR (1 mentions) Anti lamina, by Proteintech (1 mentions) Anti eif4a3, by Proteintech (1 mentions) Anti c myc, by Proteintech (1 mentions) Anti cyclin e, by Abcam (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Nitrocellulose membrane, by Beyotime (1 mentions) Anti β actin, by ABclonal (1 mentions)

3 protocols using anti tfrc

1

Antibodies and Reagents for Cell Signaling

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The following antibodies were used: anti-GAPDH (100242-MM05, Sino Biologica, China), anti-β-Actin (sc-47778, Santa Cruz Biotechnology, USA), anti-SLC7A11 (A2413, ABclonal, China, China), ati-SLC3A2 (A19880, ABclonal, China), anti-GPX4 (A11243, ABclonal, China), anti-GPX4 (A1933, ABclonal, China), anti-GPX4 (67763-1-Ig, Proteintech, USA), anti-TFRC (A5865, ABclonal, China), anti-ICAM-1 (A19300, ABclonal, China), anti-VCAM-1 (A0279, ABclonal, China), anti-IL-1β (A19636, ABclonal, China), anti-Parkin (A0968, ABclonal, China), anti-TOM20 (A19403, ABclonal, China). Neu5Ac (purity: >95%, #HY-10400) was purchased from MCE (China), apoptosis inhibitor Z-VAD-FMK (C1202-0.02ml, Beyotim, China), ferroptosis inhibitor Ferrostatin-1 (T6500, Topscience, USA), ferroptosis inducer Erastin (E1765, Topscience, USA) and P-3Fax-Neu5Ac (#5760/10) were obtained from R&D Systems (USA).
Xiang P., Chen Q., Chen L., Lei J., Yuan Z., Hu H., Lu Y., Wang X., Wang T., Yu R., Zhang W., Zhang J., Yu C, & Ma L. (2023). Metabolite Neu5Ac triggers SLC3A2 degradation promoting vascular endothelial ferroptosis and aggravates atherosclerosis progression in ApoE-/-mice. Theranostics, 13(14), 4993-5016.
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2

Western Blot Analysis of Protein Expression

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Total proteins were extracted by using RIPA lysis buffer (Beyotime, Jiangsu, China). Protein lysates were separated by 10% SDS-PAGE gels and then transferred to a nitrocellulose membrane (Beyotime, Jiangsu, China). After incubation with 5% non-fat milk for more than 1 h to block nonspecific binding, the membranes were immunoblotted overnight at 4°C with primary antibodies, i.e., anti-EIF4A3 (Proteintech, USA), anti-c-Myc (Proteintech, USA), anti-cyclin E (Abcam, USA), anti-CDK4 (Abcam, USA), anti-TFRC (ABclonal, China) anti-Lamin A (Proteintech, USA) and anti-β-actin (ABclonal, China). Then, the membranes were incubated with diluted secondary antibodies for 1 h at room temperature. The membranes were scanned by an Odyssey infrared scanning system (LI-COR Biosciences, Lincoln, NE, USA).
Wang X., Chen M, & Fang L. (2021). hsa_circ_0068631 promotes breast cancer progression through c-Myc by binding to EIF4A3. Molecular Therapy. Nucleic Acids, 26, 122-134.
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3

Antibody-Based Ferrostatin-1 Pathway Study

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Antibodies used in this study include anti-GAPDH (60004-1-Ig, Proteintech), anti-FSP1 (20886-1-AP, Proteintech), anti-GPX4 (ab125066, Abcam), anti-TFRC (A5865, ABclonal), anti-SLC7A11 (A2413, ABclonal), anti-TRIM54 (21074-1-AP, Proteintech), anti-ERK (#4695, Cell Signaling Technology), anti-ubiquitin (sc-9133, Santa Cruz Biotechnology), and horseradish peroxidase-conjugated secondary antibodies (VA001 and VA002, Vicmed). Reagents used in this study include Sorafenib (T0093L, Targetmol), Ferrostatin-1 (A4371, APE × BIO), Z-VAD-FMK (A1902, APE × BIO), Necrosulfonamide (B7731, APE × BIO), cycloheximide (C7698-1G, SIGMA), MG-132 (A2585, APE × BIO), and PD98059 (HY-12028, MedChemExpress).
Liu M.R., Shi C., Song Q.Y., Kang M.J., Jiang X., Liu H, & Pei D.S. (2023). Sorafenib induces ferroptosis by promoting TRIM54-mediated FSP1 ubiquitination and degradation in hepatocellular carcinoma. Hepatology Communications, 7(10), e0246.
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