Ddx25 sc 51271

Manufactured by Santa Cruz Biotechnology

DDX25 (sc-51271) is a lab equipment product offered by Santa Cruz Biotechnology. It is a protein that functions as an RNA helicase, which is involved in the unwinding of RNA secondary structures. This product is intended for use in research applications.

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Lab products found in correlation

2 protocols using ddx25 sc 51271

Antibody Validation for Germ Cell Markers

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Primary antibodies used in this study were: DDX25 (sc-51271) and Hiwi (sc-22685) from Santa Cruz Biotechnology; DDX4 (ab13840), EIF4A3 (ab32485), and SMG6 (ab87539) from Abcam; PIWIL1 (G82) from Cell Signaling Technology; FYCO1 (HPA035526 and SAB1400697) from Sigma-Aldrich; GAPDH (5G4) from HyTest; γH2AX antibody (05-636) and PIWIL2 clone 13E-3 (MABE363) from Millipore; SMG7 (A302-170A) from Bethyl Laboratories and hVasa (AF2030-SP) from R&D systems. Secondary antibodies conjugated with Alexa Fluor 488, 546, and 647 made in donkey were from Thermo Fisher Scientific (A-21202, A-21206, A-11055, A10036, A10040, A-11056, A-21203, A-21207, A-11058, A-31571, A-31573, A-21447). HRP-linked anti-rabbit IgG (NA934) and HRP-linked anti-mouse IgG (NA031) were from GE Healthcare Life Sciences.

Lehtiniemi T., Bourgery M., Ma L., Ahmedani A., Mäkelä M., Asteljoki J., Olotu O., Laasanen S., Zhang F.P., Tan K., Chousal J.N., Burow D., Koskinen S., Laiho A., Elo L.L., Chalmel F., Wilkinson M.F, & Kotaja N. (2022). SMG6 localizes to the chromatoid body and shapes the male germ cell transcriptome to drive spermatogenesis. Nucleic Acids Research, 50(20), 11470-11491.

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Antibody Validation for Germ Cell Analysis

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Primary antibodies used in this study were: ACTA2/α actin (sc-32251) and DDX25 (sc-51271) from Santa Cruz Biotechnology; TUBA/α tubulin (MS-581-P1) from Thermo Fisher Scientific; DDX4 (ab13840), LAMP1 (ab25245), EIF4A3 (ab32485), RUVBL2 (ab36569) and DCP1A (ab47811) from Abcam; PIWIL1 (G82) and LC3B (2775) from Cell Signaling Technology; FYCO1 (H00079443-A01) from Abnova; FYCO1 (HPA035526 and SAB1400697) and RUVBL1 (HPA019948) from Sigma-Aldrich; GAPDH (5G4) from HyTest; PIWIL2 clone 13E-3 (MABE363) from Millipore. Rabbit polyclonal antibody against TSKS was a kind gift from Prof. J.A. Grootegoed, Department of Reproduction and Development, Erasmus MC - University Medical Center Rotterdam. Secondary antibodies conjugated with Alexa Fluor 488, 546, 594 and 647 made in donkey and streptavidin conjugated with Alexa Fluor 488 or 647 were purchased from Thermo Fisher Scientific (A-21202, A-21206, A-11055, A10036, A10040, A-11056, A-21203, A-21207, A-11058, A-31571, A-31573, A-21447, S32354, S32357). ECL anti-mouse IgG HRP-linked whole antibody made in sheep (NA931) and ECL anti-rabbit IgG HRP-linked made in donkey (NA934) were purchased from GE Healthcare Life Sciences.

Da Ros M., Lehtiniemi T., Olotu O., Fischer D., Zhang F.P., Vihinen H., Jokitalo E., Sironen A., Toppari J, & Kotaja N. (2016). FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules. Autophagy, 13(2), 302-321.

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