Selected tissues, including visceral organs and nervous tissues, were collected and fixed in 20% buffered formalin for histopathological examination. For immunohistochemistry (IHC), a streptavidin-biotin-peroxidase system (SAB-PO Kit; Nichirei Bioscience, Tokyo, Japan) was employed. Primary antibodies used for IHC were monoclonal mouse anti-rabies nucleoprotein (clone N13-27; kindly provided by Dr. Naoto Ito, Gifu University), monoclonal mouse anti-human GFAP (clone 6F2; DAKO, Carpinteria, CA, USA), monoclonal mouse anti-human CD3 (clone F7.2.38; DAKO, USA), monoclonal mouse anti-human CD79α (clone MH57; DAKO), and goat polyclonal anti-rabbit Iba-1 (code ab5076; Abcam, Cambridge, UK).
Monoclonal mouse anti human cd3 clone f7.2.38
Manufactured by Agilent Technologies
Sourced in United States
Monoclonal mouse anti-human CD3 (clone F7.2.38) is a laboratory reagent used to detect the presence of CD3, a protein complex found on the surface of mature T cells. This monoclonal antibody is specific to the CD3 antigen and can be used in various immunoassay techniques to identify and quantify T cells.
Automatically generated - may contain errors
Lab products found in correlation
Sabpo kit, by Nichirei Biosciences (1 mentions)
Pillcam sb3, by Medtronic (1 mentions)
Anti pcna antibody, by Abcam (1 mentions)
Anti cd163 antibody, by Abcam (1 mentions)
Monoclonal mouse anti human cd68 clone pg m1, by Agilent Technologies (1 mentions)
Sma clone 1a4, by Agilent Technologies (1 mentions)
3 protocols using monoclonal mouse anti human cd3 clone f7.2.38
1
Histopathological and Immunohistochemical Analysis
2
Endoscopic Evaluation of Celiac Disease
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Upper gastrointestinal endoscopies and biopsies were performed with a high-definition gastroscope (Pentax, Toshima, Japan) and standard bioptic forceps (Boston Scientific, USA). Four oriented biopsy specimens were taken from the duodenum. Briefly, the samples were oriented on adhesive filter paper, fixed in a 10% formalin buffer, and paraffin-embedded. Sections were cut from each block and hematoxylin/eosin stained. Intraepithelial lymphocytes (IELs) were counted by means of anti-CD3 immunohistochemical staining (monoclonal mouse anti-human CD3 clone F7.2.38, Dako, Italy) [10 (link)]. Small bowel exploration was performed by means of video capsule endoscopy (VCE) (PillCam™ SB 3, Medtronic, Minneapolis, MN, USA). VCE was performed after intestinal cleaning with a 3-liter polyethylene-glycol (PEG)-based solution and overnight fasting. The Medtronic Imaging recording system was positioned according to the manufacturer’s instructions; data were downloaded on a dedicated computer workstation, analyzed by dedicated software, and read by an expert endoscopist [22 (link),23 (link)].
CD patients undergoing upper endoscopy and VCE were selected following the international guidelines [1 (link),24 (link),25 (link)].
CD patients undergoing upper endoscopy and VCE were selected following the international guidelines [1 (link),24 (link),25 (link)].
3
Immunohistochemical Characterization of Murine LCWE-Induced Inflammation
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Paraffinized sections (2.5 μm-thick) from LCWE-injected mice were stained with the
following antibodies: anti-mouse CD3 (1:200, monoclonal mouse anti-human CD3 clone
F7.2.38, Dako, Santa Clara, CA, USA), anti-CD20 (1:400, L26 monoclonal mouse antibody,
Nichirei Bioscience, Inc., Tokyo, Japan), anti-CD68 (1:500, monoclonal mouse anti-human
CD68 clone PG-M1, Dako), anti-CD163 (1:2000, anti-CD163 antibody, Abcam, Cambridge, UK),
α-smooth muscle actin (SMA, 1:1000, SMA clone 1A4, Dako), and anti-PCNA (1:800, anti-PCNA
antibody, Abcam). In each experiment, negative controls without the primary antibody were
included and showed no staining.
following antibodies: anti-mouse CD3 (1:200, monoclonal mouse anti-human CD3 clone
F7.2.38, Dako, Santa Clara, CA, USA), anti-CD20 (1:400, L26 monoclonal mouse antibody,
Nichirei Bioscience, Inc., Tokyo, Japan), anti-CD68 (1:500, monoclonal mouse anti-human
CD68 clone PG-M1, Dako), anti-CD163 (1:2000, anti-CD163 antibody, Abcam, Cambridge, UK),
α-smooth muscle actin (SMA, 1:1000, SMA clone 1A4, Dako), and anti-PCNA (1:800, anti-PCNA
antibody, Abcam). In each experiment, negative controls without the primary antibody were
included and showed no staining.
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