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3 protocols using isoliquiritigenin

1

Quantification of Herbal Compounds

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The reference compounds of oxypaeoniflorin, albiflorin, paeoniflorin, liquiritin, isoliquiritin, liquiritigenin, benzoylpaeoniflorin, isoliquiritigenin, and glycyrrhizic acid (purity ≥ 98%) were purchased from Chengdu Pufeide Biotech Co., Ltd. of China (Chengdu, China). Medicinal materials were purchased from Hebei anguo Chinese Herbal Medicine Co., Ltd. of China (Hebei, China). Acetic acid was purchased from Tianjin Chemical Regent Co., Ltd. (Tianjin, China), methanol and acetonitrile were HPLC-grade (Fisher, USA) and high purity water was obtained from Wahaha Co., Ltd. (Hangzhou, China).
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2

Phytochemical Anti-Inflammatory Screening

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Isoliquiritigenin, isorhamnetin and quercetin (≥98% purity by HPLC) was obtained from Chengdu Pufei De Biotech Co., Ltd (Chengdu, China). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco (Grand Island, USA). Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich Co., Ltd (St Louis, USA).
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3

Quantification of Bioactive Compounds in Medicinal Plants

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The reference standards of 6-gingerol, 8-gingerol, 6-shogaol, liquiritin, liquiritigenin, isoliquiritin, isoliquiritigenin, and glycyrrhizic acid (purity ≥ 98%) were purchased from Chengdu Pufeide Biotech Co., Ltd. (Chengdu, China). Methanol, acetonitrile (Fisher, USA), and phosphoric acid (Tianjin Chemical Regent Co., Ltd., Tianjin, China) were of HPLC grade. The distilled water was obtained from Wahaha Co., Ltd. (Hangzhou, China).
Medicinal materials were purchased from Hebei Anguo medicine market (Hebei, China) and authenticated by Professor Guijun Zhang (Beijing University of Chinese Medicine, Beijing, China).
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